Quantification of Desulfovibrio vulgaris Dissimilatory Sulfite Reductase Gene Expression during Electron Donor- and Electron Acceptor-Limited Growth

Previous studies have suggested that levels of transcripts for dsrA, a gene encoding a subunit of the dissimilatory sulfite reductase, are not directly related to the rates of sulfate reduction in sediments under all conditions. This phenomenon was further investigated with chemostat-grown Desulfovi...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2008-09, Vol.74 (18), p.5850-5853
Main Authors: Villanueva, Laura, Haveman, Shelley A, Summers, Zara M, Lovley, Derek R
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Chemostats
Desulfovibrio vulgaris
Desulfovibrio vulgaris - enzymology
Desulfovibrio vulgaris - genetics
Desulfovibrio vulgaris - metabolism
Electron Transport
Electrons
Environmental Microbiology
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes
Geologic Sediments - microbiology
Hydrogensulfite Reductase - genetics
Microbiology
Oxidation-Reduction
RNA, Bacterial - genetics
RNA, Messenger - analysis
Sediments
Sulfates - metabolism
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Summary:Previous studies have suggested that levels of transcripts for dsrA, a gene encoding a subunit of the dissimilatory sulfite reductase, are not directly related to the rates of sulfate reduction in sediments under all conditions. This phenomenon was further investigated with chemostat-grown Desulfovibrio vulgaris. Under sulfate-limiting conditions, dsrA transcript levels increased as the bulk rates of sulfate reduction in the chemostat increased, but transcript levels were similar at all sulfate reduction rates under electron donor-limiting conditions. When both electron donor- and electron acceptor-limiting conditions were considered, there was a direct correspondence between dsrA transcript levels and the rates of sulfate reduction per cell. These results suggest that dsrA transcript levels may provide important information on the metabolic state of sulfate reducers.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.00399-08