Synthesis and Uptake of the Compatible Solutes Ectoine and 5-Hydroxyectoine by Streptomyces coelicolor A3(2) in Response to Salt and Heat Stresses

• Published in: Applied and Environmental Microbiology 2008-12, Vol.74 (23), p.7286-7296
• Main Authors: Bursy, Jan, Kuhlmann, Anne U, Pittelkow, Marco, Hartmann, Holger, Jebbar, Mohamed, Pierik, Antonio J, Bremer, Erhard
• Format: Article
• Language: English
• Subjects: Amino Acids, Diamino - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Carbon Radioisotopes - metabolism; Cell culture; Fundamental and applied biological sciences. Psychology; Hot Temperature; Hydrolases - genetics; Hydrolases - isolation & purification; Hydrolases - metabolism; Life Sciences; Metabolic Networks and Pathways; Microbiology; Microbiology and Parasitology; Multigene Family; Physiology and Biotechnology; Salts - metabolism; Streptomyces; Streptomyces coelicolor - drug effects; Streptomyces coelicolor - genetics; Streptomyces coelicolor - metabolism; Streptomyces coelicolor - radiation effects
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.00768-08

Streptomyces coelicolor A3(2) synthesizes ectoine and 5-hydroxyectoine upon the imposition of either salt (0.5 M NaCl) or heat stress (39°C). The cells produced the highest cellular levels of these compatible solutes when both stress conditions were simultaneously imposed. Protection against either severe salt (1.2 M NaCl) or heat stress (39°C) or a combination of both environmental cues could be accomplished by adding low concentrations (1 mM) of either ectoine or 5-hydroxyectoine to S. coelicolor A3(2) cultures. The best salt and heat stress protection was observed when a mixture of ectoine and 5-hydroxyectoine (0.5 mM each) was provided to the growth medium. Transport assays with radiolabeled ectoine demonstrated that uptake was triggered by either salt or heat stress. The most effective transport and accumulation of [¹⁴C]ectoine by S. coelicolor A3(2) were achieved when both environmental cues were simultaneously applied. Our results demonstrate that the accumulation of the compatible solutes ectoine and 5-hydroxyectoine allows S. coelicolor A3(2) to fend off the detrimental effects of both high salinity and high temperature on cell physiology. We also characterized the enzyme (EctD) required for the synthesis of 5-hydroxyectoine from ectoine, a hydroxylase of the superfamily of the non-heme-containing iron(II)- and 2-oxoglutarate-dependent dioxygenases (EC 1.14.11). The gene cluster (ectABCD) encoding the enzymes for ectoine and 5-hydroxyectoine biosynthesis can be found in the genome of S. coelicolor A3(2), Streptomyces avermitilis, Streptomyces griseus, Streptomyces scabiei, and Streptomyces chrysomallus, suggesting that these compatible solutes play an important role as stress protectants in the genus STREPTOMYCES: