Characterization of Garvicin ML, a Novel Circular Bacteriocin Produced by Lactococcus garvieae DCC43, Isolated from Mallard Ducks (Anas platyrhynchos)

Lactococcus garvieae DCC43 produces a bacteriocin, garvicin ML (GarML), with a molecular mass of 6,004.2 Da. Data from de novo amino acid sequencing by tandem mass spectrometry and nucleotide sequencing by reverse genetics suggested that the bacteriocin is synthesized as a 63-amino-acid precursor wi...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2011-01, Vol.77 (1), p.369-373
Main Authors: Borrero, Juan, Brede, Dag A, Skaugen, Morten, Diep, Dzung B, Herranz, Carmen, Nes, Ingolf F, Cintas, Luis M, Hernández, Pablo E
Format: Article
Language:English
Subjects:
Amino acids
Anas platyrhynchos
Animals
Bacteria
Bacteriocins - chemistry
Bacteriocins - genetics
Bacteriocins - metabolism
Biological and medical sciences
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Ducks - microbiology
Fundamental and applied biological sciences. Psychology
Genetic linkage
Genetics
Genetics and Molecular Biology
Lactococcus - isolation & purification
Lactococcus - metabolism
Lactococcus garvieae
Mass Spectrometry
Microbiology
Molecular Sequence Data
Molecular Weight
Peptides
Sequence Analysis, DNA
Wildfowl
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Summary:Lactococcus garvieae DCC43 produces a bacteriocin, garvicin ML (GarML), with a molecular mass of 6,004.2 Da. Data from de novo amino acid sequencing by tandem mass spectrometry and nucleotide sequencing by reverse genetics suggested that the bacteriocin is synthesized as a 63-amino-acid precursor with a 3-amino-acid leader peptide that is removed by cleavage. Subsequently, a covalent linkage between the N and C termini forms the mature version of this novel 60-amino-acid circular bacteriocin.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.01173-10