Induction of proliferative responses of T cells from Babesia bovis-immune cattle with a recombinant 77-kilodalton merozoite protein (Bb-1)

A major portion of a Babesia bovis-specific gene encoding a 77-kDa merozoite protein (Bb-1) produced during natural infection in cattle and in microaerophilous culture was subcloned into the pGEX1N expression vector. Recombinant Bb-1 protein fused to glutathione S-transferase (Bb-1-GST) was used to...

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Bibliographic Details
Published in:Infection and Immunity 1992-02, Vol.60 (2), p.644-652
Main Authors: Tetzlaff, C.L. (Texas AandM University, College Station, TX), Rice-Ficht, A.C, Woods, V.M, Brown, W.C
Format: Article
Language:English
Subjects:
Animal protozoal diseases
Animals
ANTIGENE
ANTIGENOS
BABESIA BOVIS
Babesia bovis - immunology
Biological and medical sciences
BOVIN
Cattle
Female
GANADO BOVINO
GENE
GENES
Glutathione Transferase - immunology
IMMUNITE CELLULAIRE
Immunization
Infectious diseases
INMUNIDAD CELULAR
LINFOCITOS
LYMPHOCYTE
Lymphocyte Activation
Medical sciences
Parasitic diseases
PROTEINAS
PROTEINE
Protozoal diseases
Protozoan Proteins - immunology
Rabbits
RECOMBINACION
RECOMBINAISON
Recombinant Fusion Proteins - analysis
Recombinant Fusion Proteins - immunology
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
T-Lymphocytes - immunology
Vaccines, Synthetic - immunology
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Summary:A major portion of a Babesia bovis-specific gene encoding a 77-kDa merozoite protein (Bb-1) produced during natural infection in cattle and in microaerophilous culture was subcloned into the pGEX1N expression vector. Recombinant Bb-1 protein fused to glutathione S-transferase (Bb-1-GST) was used to examine cellular immune responses in B. bovis-immune cattle. Sera from rabbits immunized with Bb-1-GST reacted with fusion protein and with the native antigen present in crude B. bovis but not with B. bigemina merozoites. Bb-1-GST but not GST induced strong proliferation of T lymphocytes from these immune cattle, and Bb-1-reactive T-cell lines which consisted of a mixed population of either CD4+ and CD8+ cells or CD4+, CD8+, and "null" (gamma-delta T) cells were established by in vitro stimulation of peripheral blood mononuclear cells with the recombinant fusion protein. Three CD4+ CD8- and three CD4- CD8+ Bb-1-specific T-cell clones were identified after limiting-dilution cloning of the cell lines. The studies described here demonstrate that the 77-kDa protein of B. bovis contains T-cell epitopes capable of eliciting proliferation of two types of T cells in immune cattle, an important consideration for the design of a recombinant subunit vaccine
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.60.2.644-652.1992