Loading…

Cre Reporter System To Monitor the Translocation of Type III Secreted Proteins into Host Cells

Central to the study of type III secretion systems is the availability of reporter systems to monitor bacterial protein translocation into host cells. We report here the development of a bacteriophage P1 Cre recombinase-based system to monitor the translocation of bacterial proteins into mammalian c...

Full description

Saved in:
Bibliographic Details
Published in:Infection and Immunity 2006-02, Vol.74 (2), p.1084-1090
Main Authors: Briones, Gabriel, Hofreuter, Dirk, Galán, Jorge E
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Central to the study of type III secretion systems is the availability of reporter systems to monitor bacterial protein translocation into host cells. We report here the development of a bacteriophage P1 Cre recombinase-based system to monitor the translocation of bacterial proteins into mammalian cells. Bacteriophage P1 Cre recombinase fused to the secretion and translocation signals of Salmonella enterica serovar Typhimurium of the type III secreted protein SopE was secreted in a type III secretion system-dependent fashion. More importantly, the SopE-Cre chimera was translocated into host cells via the type III secretion system and activated the expression of luciferase and green fluorescent protein reporters of Cre recombinase activity.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.74.2.1084-1090.2006