pepM Is an essential gene in Salmonella typhimurium

The pepM gene of Salmonella typhimurium codes for a methionine-specific aminopeptidase that removes N-terminal methionine residues from proteins. This gene was inactivated in vitro by the insertion of a DNA fragment coding for kanamycin resistance. The inactivated gene could not replace the wild-typ...

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Bibliographic Details
Published in:Journal of Bacteriology 1989-09, Vol.171 (9), p.5215-5217
Main Authors: Miller, C.G. (Case Western Reserve University, Cleveland, OH), Kukral, A.M, Miller, J.L, Rao Movva, N
Format: Article
Language:English
Subjects:
Aminopeptidases - genetics
Bacteriology
Biological and medical sciences
Blotting, Southern
Crosses, Genetic
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Bacterial
HIDROLASAS
HYDROLASE
Metabolism. Enzymes
Methionyl Aminopeptidases
Microbiology
Nucleic Acid Hybridization
Plasmids
SALMONELLA
Salmonella typhimurium
Salmonella typhimurium - enzymology
Salmonella typhimurium - genetics
Transduction, Genetic
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Description
Summary:The pepM gene of Salmonella typhimurium codes for a methionine-specific aminopeptidase that removes N-terminal methionine residues from proteins. This gene was inactivated in vitro by the insertion of a DNA fragment coding for kanamycin resistance. The inactivated gene could not replace the wild-type chromosomal pepM gene unless another functional copy was present in the cell. The lethal effect of the pepM insertion was not a result of polarity on any gene downstream, nor was it affected by the presence or absence of other peptidases
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.171.9.5215-5217.1989