Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism

• Published in: Journal of Bacteriology 1990-03, Vol.172 (3), p.1628-1633
• Main Authors: Kuske, C.R. (Los Alamos National Laboratory, Los Alamos, NM), Kirkpatrick, B.C
• Format: Article
• Language: English
• Subjects: Blotting, Southern; Chromosomes, Bacterial; DNA - isolation & purification; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; ENFERMEDADES DE LAS PLANTAS; IDENTIFICACION; IDENTIFICATION; MALADIE DES PLANTES; MICOPLASMA; Mycoplasma - genetics; Mycoplasma - isolation & purification; MYCOPLASME; Nucleic Acid Hybridization; Plants - genetics; Plants - microbiology; PLASMIDE; PLASMIDIOS; Plasmids
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.172.3.1628-1633.1990

Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO) as the probe, identified at least four plasmids in celery, aster, and periwinkle plants and in Macrosteles severini leafhopper vectors infected with either the dwarf AY-MLO, Tulelake AY-MLO, or SAY-MLO strain. Plasmids were also detected in two California field isolates of AY-MLO but not in plants infected with the beet leafhopper-transmitted virescence agent, western X, or elm yellows MLOs. SAY-MLO plasmids were 5.2, 4.9, 3.4, and 1.7 kilobase pairs in size. Plasmids isolated from dwarf AY- and Tulelake AY-MLOs were 7.4, 5.1, 3.5, and 1.7 kilobase pairs in size. No evidence was obtained for integration of SAY-MLO plasmids into the MLO chromosome