Cloning, expression, and characterization of the Lactococcus lactis pfl gene, encoding pyruvate formate-lyase

The Lactococcus lactis pfl gene, encoding pyruvate formate-lyase (PFL), has been cloned and characterized. The deduced amino acid sequence of the L. lactis PFL protein showed high similarity to those of other bacterial PFL proteins and included the conserved glycine residue involved in posttranslati...

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Bibliographic Details
Published in:Journal of Bacteriology 1997-09, Vol.179 (18), p.5884-5891
Main Authors: Arnau, J, Jorgensen, F, Madsen, S.M, Vrang, A, Israelsen, H
Format: Article
Language:English
Subjects:
Acetyltransferases - genetics
ACIDE FORMIQUE
ACIDO FORMICO
ACILTRANSFERASA
ACYLTRANSFERASE
ACYLTRANSFERASES
Amino Acid Sequence
AMINO ACID SEQUENCES
ANAEROBIC CONDITIONS
ANAEROBIOSE
ANAEROBIOSIS
Bacteriology
Base Sequence
CHEMICAL COMPOSITION
CLONACION
CLONAGE
CLONING
Cloning, Molecular
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
EXPRESION GENICA
EXPRESSION DES GENES
FORMIC ACID
GALACTOSA
GALACTOSE
GENBANK/AJ000325
GENBANK/AJ000326
GENE
GENE EXPRESSION
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
GENES
Genes, Bacterial
Genetics
LACTOCOCCUS LACTIS
Lactococcus lactis - genetics
LACTOCOCCUS LACTIS SUBSP. CREMORIS
LACTOCOCCUS LACTIS SUBSP. LACTIS
MOLECULAR SEQUENCE DATA
NUCLEOTIDE SEQUENCE
OPEN READING FRAMES
PROMOTERS
Proteins
Restriction Mapping
SECUENCIA NUCLEOTIDICA
Sequence Alignment
Sequence Homology, Amino Acid
SEQUENCE NUCLEOTIDIQUE
Species Specificity
STRUCTURAL GENES
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Description
Summary:The Lactococcus lactis pfl gene, encoding pyruvate formate-lyase (PFL), has been cloned and characterized. The deduced amino acid sequence of the L. lactis PFL protein showed high similarity to those of other bacterial PFL proteins and included the conserved glycine residue involved in posttranslational activation of PFL. The genetic organization of the chromosomal pfl region in L. lactis showed differences from other characterized pfl loci, with an upstream open reading frame independently transcribed in the same orientation as the pfl gene. The gene coding for PFL-activase (act), normally found downstream of pfl, was not identified in L. lactis. Analysis of pfl expression showed a strong induction under anaerobiosis at the transcriptional level independent of the growth medium used. During growth with galactose, pfl showed the highest levels of expression. Constructed L. lactis pfl strains were unable to produce formate under anaerobic growth. Higher levels of diacetyl and acetoin were produced anaerobically in the constructed Lactococcus lactis subsp. lactis biovar diacetylactis pfl strain
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.179.18.5884-5891.1997