Identification of subgroups of bovine respiratory syncytial virus

The occurrence of antigenic variation among nine isolates of bovine respiratory syncytial virus (BRSV) was determined by examining their reaction patterns to human respiratory syncytial virus (HRSV) subgroup A and B monoclonal antibodies (MAbs) by enzyme immunoassay and radioimmunoprecipitation with...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Clinical Microbiology 1992-05, Vol.30 (5), p.1120-1126
Main Authors: Baker, J.C. (Michigan State University, East Lansing, MI), Wilson, E.G, McKay, G.L, Stanek, R.J, Underwood, W.J, Velicer, L.F, Mufson, M.A
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
ANTIGENE
ANTIGENOS
Antigens, Viral - analysis
Biological and medical sciences
BOVIN
Cattle
Fundamental and applied biological sciences. Psychology
GANADO BOVINO
GENERO HUMANO
GENRE HUMAIN
HN Protein
Humans
Immunoenzyme Techniques
Microbiology
PNEUMOVIRUS
Precipitin Tests
PROTEINAS
PROTEINE
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Respiratory Syncytial Viruses - classification
Respiratory Syncytial Viruses - immunology
Respiratory Syncytial Viruses - isolation & purification
Viral Envelope Proteins
Viral Proteins
Viral Vaccines - immunology
Virology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The occurrence of antigenic variation among nine isolates of bovine respiratory syncytial virus (BRSV) was determined by examining their reaction patterns to human respiratory syncytial virus (HRSV) subgroup A and B monoclonal antibodies (MAbs) by enzyme immunoassay and radioimmunoprecipitation with fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by using MAbs and polyclonal antisera to HRSV and BRSV. Shared epitopes were demonstrated on four of five structural proteins between BRSV and both subgroups A and B of HRSV. The nine isolates of BRSV showed different patterns of reactivity in enzyme immunoassays with panels of MAbs to HRSV subgroups A and B. Major variations in the molecular weights of the P (phosphoprotein) and F (fusion protein) proteins were demonstrated among the BRSV isolates tested. These results suggest that BRSV belongs to a different antigenic grouping than HRSV and that BRSV is composed of two distinct subgroups
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.30.5.1120-1126.1992