Serotype identification of Actinobacillus pleuropneumoniae by arbitrarily primed polymerase chain reaction

Rapid and accurate determination of the Actinobacillus pleuropneumoniae serotype involved in a disease outbreak is important both in limiting the severity of an outbreak and for tracing the source of the infecting organism. This study describes the use of arbitrarily primed polymerase chain reaction...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1993-05, Vol.31 (5), p.1155-1159
Main Authors: Hennessy, K.J. (Kansas State University, Manhattan, KS), Landolo, J.J, Fenwick, B.W
Format: Article
Language:English
Subjects:
Actinobacillus Infections - microbiology
Actinobacillus Infections - veterinary
ACTINOBACILLUS PLEUROPNEUMONIAE
Actinobacillus pleuropneumoniae - classification
Actinobacillus pleuropneumoniae - genetics
Animals
Bacterial Typing Techniques
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
BIOCHIMIE
Biological and medical sciences
BIOQUIMICA
DNA, Bacterial - genetics
Evaluation Studies as Topic
Fundamental and applied biological sciences. Psychology
Microbiology
Molecular Sequence Data
Polymerase Chain Reaction
SEROTIPOS
SEROTYPE
Serotyping
Swine
Swine Diseases - microbiology
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Summary:Rapid and accurate determination of the Actinobacillus pleuropneumoniae serotype involved in a disease outbreak is important both in limiting the severity of an outbreak and for tracing the source of the infecting organism. This study describes the use of arbitrarily primed polymerase chain reaction (AP-PCR) as a rapid, precise, and genetically based procedure to identify A. pleuropneumoniae. AP-PCR amplification of bacterial genomic DNA results in specific DNA profiles, which can be used to differentiate currently recognized serotypes. This technique is especially useful for identifying previously nontypeable and serologically cross-reactive A. pleuropneumoniae field isolates. Consecutive passages of isolates on different media, freezing, and subsequent infection of pigs did not alter the AP-PCR genomic profile. We propose the use of M13 and T3-T7 oligodeoxynucleotide primers for diagnostic and epidemiological identification of A. pleuropneumoniae by AP-PCR techniques
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.31.5.1155-1159.1993