Prevalence of Bartonella species in domestic cats in The Netherlands

Cats have been shown to provide the only known reservoir of Bartonella henselae, the causative agent of cat scratch disease. To determine the prevalence of Bartonella bacteremia and antibodies in Dutch cats, blood samples from 113 cats from shelters (sheltered cats), 50 pet cats, and 25 specific-pat...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1997-09, Vol.35 (9), p.2256-2261
Main Authors: Bergmans, A.M.C. (National Institute of Public Health and the Environment, Bilthoven, The Netherlands.), Jong, C.M.A. de, Amerongen, G. van, Schot, C.S, Schouls, L.M
Format: Article
Language:English
Subjects:
Animal bacterial diseases
Animals
Antibodies, Bacterial - analysis
Bacteremia - diagnosis
Bacterial diseases
Bacteriology
Bartonella - immunology
Bartonella - isolation & purification
Biological and medical sciences
Cat-Scratch Disease - blood
Cat-Scratch Disease - epidemiology
Cat-Scratch Disease - veterinary
CATS
Cats - microbiology
CHAT
Epidemiology
Female
Fundamental and applied biological sciences. Psychology
GATO
Infectious diseases
Male
Medical sciences
Microbiology
Molecular Epidemiology
Molecular Sequence Data
NETHERLANDS
Netherlands - epidemiology
PAISES BAJOS
PAYS BAS
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prevalence
RICKETTSIALES
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Seroepidemiologic Studies
Siphonaptera - microbiology
Specific Pathogen-Free Organisms
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Summary:Cats have been shown to provide the only known reservoir of Bartonella henselae, the causative agent of cat scratch disease. To determine the prevalence of Bartonella bacteremia and antibodies in Dutch cats, blood samples from 113 cats from shelters (sheltered cats), 50 pet cats, and 25 specific-pathogen-free (SPF) cats were analyzed. Culture and subsequent PCR-restriction fragment length polymorphism (RFLP) analysis of the 16S-23S rRNA intergenic region and 16S rRNA gene PCR-hybridization assays revealed a prevalence of Bartonella bacteremia in 22% of the sheltered cats and showed no bacteremia in the SPF cats. Three spacer RFLP types were found: types A, B, and G, with type B being predominant over types A and G. An important finding was the existence of mixtures of different Bartonella species. Bartonella DNA was detected in 7 of 27 DNA extracts from fleas combed from the sheltered cats (26%). Seropositivity was 50% for sheltered cats and 56% for pet cats, as determined by a B. henselae enzyme-linked immunoassay
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.35.9.2256-2261.1997