Development of a Novel Protein Microarray Method for Serotyping Salmonella enterica Strains

An antibody microarray assay was developed for Salmonella serotyping based on the Kauffmann-White scheme. A model (8 by 15) array was constructed using 35 antibodies for identification of 20 common Salmonella serovars and evaluated using 117 target and 73 nontarget Salmonella strains. The assay allo...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-07, Vol.43 (7), p.3427-3430
Main Authors: Cai, H. Y, Lu, L, Muckle, C. A, Prescott, J. F, Chen, S
Format: Article
Language:English
Subjects:
Antibodies, Bacterial - immunology
Antigens, Bacterial - analysis
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Flagella - immunology
Fundamental and applied biological sciences. Psychology
Humans
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
O Antigens - analysis
Protein Array Analysis - instrumentation
Protein Array Analysis - methods
Salmonella enterica - classification
Serotyping
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Summary:An antibody microarray assay was developed for Salmonella serotyping based on the Kauffmann-White scheme. A model (8 by 15) array was constructed using 35 antibodies for identification of 20 common Salmonella serovars and evaluated using 117 target and 73 nontarget Salmonella strains. The assay allowed complete serovar identification of 86 target strains and partial identification of 30 target strains and allowed exclusion of the 73 nontarget strains from the target serovars.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.7.3427-3430.2005