Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA

rRNA gene sequences were used for identification and target adequacy controls in a DNA probe assay to identify isolates as Staphylococcus and, more specifically, as S. aureus within 1 hour. mecA status was simultaneously determined using a specific DNA probe. The target adequacy control guarded agai...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-07, Vol.43 (7), p.3438-3442
Main Authors: Kaplan, Shannon, Marlowe, Elizabeth M, Hogan, James J, Doymaz, Mehmet, Bruckner, David A, Simor, Andrew E
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Coagulase - metabolism
DNA Probes
Fundamental and applied biological sciences. Psychology
Genes, rRNA
Humans
Infectious diseases
Medical sciences
Methicillin Resistance - genetics
Microbiology
Miscellaneous
Penicillin-Binding Proteins
Polymerase Chain Reaction
Sensitivity and Specificity
Staphylococcal Infections - microbiology
Staphylococcus aureus - classification
Staphylococcus aureus - drug effects
Staphylococcus aureus - enzymology
Staphylococcus aureus - genetics
Time Factors
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Summary:rRNA gene sequences were used for identification and target adequacy controls in a DNA probe assay to identify isolates as Staphylococcus and, more specifically, as S. aureus within 1 hour. mecA status was simultaneously determined using a specific DNA probe. The target adequacy control guarded against false-negative mecA results.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.7.3438-3442.2005