Molecular Diagnosis of Bordetella pertussis Infection by Evaluation of Formalin-Fixed Tissue Specimens

Formalin-fixed lung or trachea tissue specimens from four infants and one adolescent who died of respiratory illness were tested for Bordetella pertussis by conventional and real-time PCR assays. B. pertussis was confirmed in all cases. PCR can be an invaluable retrospective diagnostic tool for eval...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2006-03, Vol.44 (3), p.1074-1076
Main Authors: Tatti, Kathleen M, Wu, Kai-Hui, Sanden, Gary N, Greer, Patricia, Sumner, John, Guarner, Jeannette, Paddock, Christopher D, Zaki, Sherif R
Format: Article
Language:English
Subjects:
Adolescent
Bacterial diseases
Bacteriology
Base Sequence
Biological and medical sciences
Bordetella pertussis
Bordetella pertussis - genetics
Bordetella pertussis - isolation & purification
DNA, Bacterial - genetics
Ent and stomatologic bacterial diseases
Fixatives
Formaldehyde
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Humans
Infant
Infectious diseases
Lung - microbiology
Medical sciences
Microbiology
Miscellaneous
Polymerase Chain Reaction
Retrospective Studies
Tissue Fixation
Whooping Cough - diagnosis
Whooping Cough - microbiology
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Summary:Formalin-fixed lung or trachea tissue specimens from four infants and one adolescent who died of respiratory illness were tested for Bordetella pertussis by conventional and real-time PCR assays. B. pertussis was confirmed in all cases. PCR can be an invaluable retrospective diagnostic tool for evaluating archival tissues from patients with suspected fatal pertussis.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.44.3.1074-1076.2006