Comparison of the Phenotyping Methods ID 32E and VITEK 2 Compact GN with 16S rRNA Gene Sequencing for the Identification of Enterobacter sakazakii

A total of 34 isolates (28 Enterobacter sakazakii and 6 Enterobacteriaceae) from infant formulae, milk powder, and the production environment of milk powder factories were identified using ID 32E and VITEK 2 compact GN systems (bioMérieux, France). The ID 32E version 3.0 and VITEK 2 compact GN versi...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2007-06, Vol.45 (6), p.2048-2050
Main Authors: Fanjat, Nadège, Leclercq, Alexandre, Joosten, Han, Robichon, Denis
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques - methods
Bacteriology
Biological and medical sciences
Cronobacter sakazakii - classification
Cronobacter sakazakii - genetics
Cronobacter sakazakii - physiology
Enterobacter sakazakii
Enterobacteriaceae
Food Contamination
Food Microbiology
Fundamental and applied biological sciences. Psychology
Genes, rRNA
Humans
Infant Formula
Infectious diseases
Medical sciences
Microbiology
Miscellaneous
Molecular Sequence Data
Phenotype
Polymerase Chain Reaction
Powders
Reagent Kits, Diagnostic
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Software
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Summary:A total of 34 isolates (28 Enterobacter sakazakii and 6 Enterobacteriaceae) from infant formulae, milk powder, and the production environment of milk powder factories were identified using ID 32E and VITEK 2 compact GN systems (bioMérieux, France). The ID 32E version 3.0 and VITEK 2 compact GN version 01.01b correctly identified 100% (28) of the Enterobacter sakazakii isolates tested, whereas the previous software version 2.0 for ID 32E showed only 71.4% correct results. None of the non-E. sakazakii isolates tested were misidentified as E. sakazakii with either of the identification systems used.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.00961-06