Evaluation of MeningoFinder, a Novel Multiplex Ligation-Dependent Probe Amplification Assay for Simultaneous Detection of Six Virus Species Causing Central Nervous System Infections

A multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species was developed and tested on clinical cerebrospinal fluid (CSF) samples. The assay, termed MeningoFinder, showed an accordance of 97%, concordance of 96%, interlaboratory sensitivity of 90%, and i...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2009-08, Vol.47 (8), p.2620-2622
Main Authors: Wolffs, P.F.G, Vink, C, Keijdener, J, Habek, B, Reijans, M, Simons, G, Bruggeman, C.A, van den Brule, A.J.C
Format: Article
Language:English
Subjects:
Bacteriological Techniques - methods
Biological and medical sciences
Central Nervous System Infections - virology
Cerebrospinal Fluid - virology
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Molecular Diagnostic Techniques - methods
Nucleic Acid Amplification Techniques - methods
Reproducibility of Results
Sensitivity and Specificity
Virology
Virus Diseases - diagnosis
Viruses - genetics
Viruses - isolation & purification
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Summary:A multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species was developed and tested on clinical cerebrospinal fluid (CSF) samples. The assay, termed MeningoFinder, showed an accordance of 97%, concordance of 96%, interlaboratory sensitivity of 90%, and interlaboratory specificity of 94% compared to PCRs.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.02436-08