Comparison of Two Mycoplasma genitalium Real-Time PCR Detection Methodologies

Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quanti...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2011-03, Vol.49 (3), p.1140-1142
Main Authors: Twin, Jimmy, Taylor, Nicole, Garland, Suzanne M, Hocking, Jane S, Walker, Jennifer, Bradshaw, Catriona S, Fairley, Christopher K, Tabrizi, Sepehr N
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - genetics
Bacteriological Techniques - methods
Bacteriology
Biological and medical sciences
Clinical Laboratory Techniques - methods
Female
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Miscellaneous
Mycoplasma genitalium
Mycoplasma genitalium - genetics
Mycoplasma genitalium - isolation & purification
Mycoplasma Infections - diagnosis
Mycoplasma Infections - microbiology
Polymerase Chain Reaction - methods
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
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Summary:Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.02328-10