T Is Not a Simple G-actin Sequestering Protein and Interacts with F-actin at High Concentration

Thymosin β is acknowledged as a major G-actin binding protein maintaining a pool of unassembled actin in motile vertebrate cells. We have examined the function of Tβ in actin assembly in the high range of concentrations (up to 300 μM) at which Tβ is found in highly motile blood cells. Tβ behave...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1996-04, Vol.271 (16), p.9231
Main Authors: Marie-France Carlier, Dominique Didry, Inge Erk, Jean Lepault, Marleen L. Van Troys, Joël Vandekerckhove, Irina Perelroizen, Helen Yin, Yukio Doi, Dominique Pantaloni
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thymosin β is acknowledged as a major G-actin binding protein maintaining a pool of unassembled actin in motile vertebrate cells. We have examined the function of Tβ in actin assembly in the high range of concentrations (up to 300 μM) at which Tβ is found in highly motile blood cells. Tβ behaves as a simple G-actin sequestering protein only in a range of low concentrations (
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.271.16.9231