Target Cell Susceptibility to Lysis by Human Natural Killer Cells Is Augmented by α(1,3)-Galactosyltransferase and Reduced by α(1,2)-Fucosyltransferase

Susceptibility of porcine endothelial cells to human natural killer (NK) cell lysis was found to reflect surface expression of ligands containing Gal α(1,3)GlcNAc, the principal antigen on porcine endothelium recognized by xenoreactive human antibodies. Genetically modifying expression of this epit...

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Bibliographic Details
Published in:The Journal of biological chemistry 1999-04, Vol.274 (16), p.10717
Main Authors: John H. Artrip, Pawel Kwiatkowski, Robert E. Michler, Shu-Feng Wang, Sorina Tugulea, Jan Ankersmit, Larisa Chisholm, Ian F. C. McKenzie, Mauro S. Sandrin, Silviu Itescu
Format: Article
Language:English
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Summary:Susceptibility of porcine endothelial cells to human natural killer (NK) cell lysis was found to reflect surface expression of ligands containing Gal α(1,3)GlcNAc, the principal antigen on porcine endothelium recognized by xenoreactive human antibodies. Genetically modifying expression of this epitope on porcine endothelium by transfection with the α(1,2)-fucosyltransferase gene reduced susceptibility to human NK lysis. These results indicate that surface carbohydrate remodeling profoundly affects target cell susceptibility to NK lysis, and suggest that successful transgenic strategies to limit xenograft rejection by NK cells and xenoreactive antibodies will need to incorporate carbohydrate remodeling.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.274.16.10717