Recruitment of Transcription Complexes to the β-Globin Gene Locus in Vivo and in Vitro

• Published in: The Journal of biological chemistry 2004-11, Vol.279 (48), p.50350
• Main Authors: Karen F. Vieira, Padraic P. Levings, Meredith A. Hill, Valerie J. Crusselle, Sung-Hae Lee Kang, James Douglas Engel, Jörg Bungert
• Format: Article
• Language: English
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M408883200

Erythroid-specific, high level expression of the β-globin genes is regulated by the locus control region (LCR), composed of multiple DNase I-hypersensitive sites and located far upstream of the genes. Recent studies have shown that LCR core elements recruit RNA polymerase II (pol II). In the present study we demonstrate the following: 1) pol II and other basal transcription factors are recruited to LCR core hypersensitive elements; 2) pol II dissociates from and re-associates with the globin gene locus during replication; 3) pol II interacts with the LCR but not with the β-globin gene prior to erythroid differentiation in embryonic stem cells; and 4) the erythroid transcription factor NF-E2 facilitates the transfer of pol II from immobilized LCR constructs to a β-globin gene in vitro . The data are consistent with the hypothesis that the LCR serves as the primary attachment site for the recruitment of macromolecular complexes involved in chromatin structure alterations and transcription of the globin genes.