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Effects of Nonsteroidal Anti-Inflammatory Agents (NSAIDs) on Ovarian Carcinoma Cell Lines
Purpose : Nonsteroidal anti-inflammatory agents may inhibit carcinogenesis in specific tissues including the colon, breast, and pancreas, and, hence, may prove to be effective chemopreventive agents. The purpose of this study was to investigate the cellular effects of acetylsalicylic acid (ASA), ace...
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Published in: | Clinical cancer research 2002-01, Vol.8 (1), p.202 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
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Summary: | Purpose : Nonsteroidal anti-inflammatory agents may inhibit carcinogenesis in specific tissues including the colon, breast, and pancreas,
and, hence, may prove to be effective chemopreventive agents. The purpose of this study was to investigate the cellular effects
of acetylsalicylic acid (ASA), acetaminophen, and a COX-2 inhibitor (NS-398) on the growth of cell lines of human ovarian
cancer in vitro . Experimental Design : SK-OV-3, Caov-3, and NIH:OVCAR-3 ovarian carcinoma cell lines were treated with ASA (10 −6 m —10 −2 m ), acetaminophen (10 −6 m —10 −2 m ), and a COX-2 inhibitor (10 −6 m –10 −4 m ) for 96 h. The number of viable cells was determined using a tetrazolium conversion assay. Immunohistochemical assessment
was performed for alterations in expression of Ki-67, erbB-2, COX enzyme, and apoptosis in primary ovarian cancer cells using
terminal deoxynucleotidyl transferase (Tdt)-mediated nick end labeling assay. Results : A decrease in cell number compared with controls was observed for all of the cell lines treated with ASA, acetaminophen,
and COX-2 inhibitor by cell count and tetrazolium conversion assay. A significant decrease in Ki-67 compared with controls
in the OVCAR-3 ( P = 0.005) and SK-OV-3 ( P = 0.007) cell lines after treatment with the COX-2 inhibitor was observed. We observed a decrease in mitotic activity compared
with controls in each cell line after treatment with the COX-2 inhibitor. Apoptosis was observed in primary ovarian cancer
cell culture treated with COX-2 inhibitor. Conclusion : Our results suggest additional study for the use of nonsteroidal anti-inflammatory agents, specifically COX-2 inhibitors,
as a strategy of chemoprevention for ovarian cancer. |
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ISSN: | 1078-0432 1557-3265 |