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Intestinal ischemia and reperfusion injury in transgenic mice overexpressing copper-zinc superoxide dismutase
D. R. Deshmukh, O. Mirochnitchenko, V. S. Ghole, D. Agnese, P. C. Shah, M. Reddell, R. E. Brolin and M. Inouye Department of Surgery, University of Medicine and Dentistry of New Jersey, New Brunswick 08903-0019, USA. Superoxide dismutase (SOD) scavenges oxygen radicals that are implicated in the pat...
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| Published in: | American Journal of Physiology: Cell Physiology 1997-10, Vol.273 (4), p.C1130-C1135 |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c493t-a319215fdb9ef41431e2bcd52c9b5f52847ec5eea9373f4d7af3aded227b31573 |
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| cites | cdi_FETCH-LOGICAL-c493t-a319215fdb9ef41431e2bcd52c9b5f52847ec5eea9373f4d7af3aded227b31573 |
| container_end_page | C1135 |
| container_issue | 4 |
| container_start_page | C1130 |
| container_title | American Journal of Physiology: Cell Physiology |
| container_volume | 273 |
| creator | Deshmukh, Devendra R Mirochnitchenko, Oleg Ghole, Vikram S Agnese, Doreen Shah, Pritesh C Reddell, Michael Brolin, Robert E Inouye, Masayori |
| description | D. R. Deshmukh, O. Mirochnitchenko, V. S. Ghole, D. Agnese, P. C. Shah, M. Reddell, R. E. Brolin and M. Inouye
Department of Surgery, University of Medicine and Dentistry of New Jersey, New Brunswick 08903-0019, USA.
Superoxide dismutase (SOD) scavenges oxygen radicals that are implicated in
the pathogenesis of intestinal ischemia-reperfusion injury. The effect of
intestinal ischemia and reperfusion was investigated in transgenic mice
overexpressing human Cu-Zn SOD. Ischemia was induced by occluding the
superior mesenteric artery. Myeloperoxidase activity was determined as an
index of neutrophil infiltration, and malondialdehyde levels were measured
as an indicator of lipid peroxidation. Forty-five minutes of intestinal
ischemia followed by 4 h of reperfusion caused an increase in intestinal
levels of malondialdehyde in both nontransgenic and transgenic mice, but
the concentration of malondialdehyde was significantly greater in
nontransgenic mice. Intestinal ischemia-reperfusion also caused an increase
in intestinal and pulmonary myeloperoxidase activity in nontransgenic and
transgenic mice, but the transgenic mice had significantly lower levels of
myeloperoxidase activity than nontransgenic mice. Transgenic mice had
higher levels of intestinal SOD activity than nontransgenic mice. There
were no significant differences in the catalase or glutathione peroxidase
activities. In conclusion, our study demonstrates that the overexpression
of SOD protects tissues from neutrophil infiltration and lipid peroxidation
during intestinal ischemia-reperfusion. |
| doi_str_mv | 10.1152/ajpcell.1997.273.4.c1130 |
| format | article |
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Department of Surgery, University of Medicine and Dentistry of New Jersey, New Brunswick 08903-0019, USA.
Superoxide dismutase (SOD) scavenges oxygen radicals that are implicated in
the pathogenesis of intestinal ischemia-reperfusion injury. The effect of
intestinal ischemia and reperfusion was investigated in transgenic mice
overexpressing human Cu-Zn SOD. Ischemia was induced by occluding the
superior mesenteric artery. Myeloperoxidase activity was determined as an
index of neutrophil infiltration, and malondialdehyde levels were measured
as an indicator of lipid peroxidation. Forty-five minutes of intestinal
ischemia followed by 4 h of reperfusion caused an increase in intestinal
levels of malondialdehyde in both nontransgenic and transgenic mice, but
the concentration of malondialdehyde was significantly greater in
nontransgenic mice. Intestinal ischemia-reperfusion also caused an increase
in intestinal and pulmonary myeloperoxidase activity in nontransgenic and
transgenic mice, but the transgenic mice had significantly lower levels of
myeloperoxidase activity than nontransgenic mice. Transgenic mice had
higher levels of intestinal SOD activity than nontransgenic mice. There
were no significant differences in the catalase or glutathione peroxidase
activities. In conclusion, our study demonstrates that the overexpression
of SOD protects tissues from neutrophil infiltration and lipid peroxidation
during intestinal ischemia-reperfusion.</description><identifier>ISSN: 0363-6143</identifier><identifier>EISSN: 1522-1563</identifier><identifier>DOI: 10.1152/ajpcell.1997.273.4.c1130</identifier><identifier>PMID: 9357755</identifier><language>eng</language><ispartof>American Journal of Physiology: Cell Physiology, 1997-10, Vol.273 (4), p.C1130-C1135</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-a319215fdb9ef41431e2bcd52c9b5f52847ec5eea9373f4d7af3aded227b31573</citedby><cites>FETCH-LOGICAL-c493t-a319215fdb9ef41431e2bcd52c9b5f52847ec5eea9373f4d7af3aded227b31573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Deshmukh, Devendra R</creatorcontrib><creatorcontrib>Mirochnitchenko, Oleg</creatorcontrib><creatorcontrib>Ghole, Vikram S</creatorcontrib><creatorcontrib>Agnese, Doreen</creatorcontrib><creatorcontrib>Shah, Pritesh C</creatorcontrib><creatorcontrib>Reddell, Michael</creatorcontrib><creatorcontrib>Brolin, Robert E</creatorcontrib><creatorcontrib>Inouye, Masayori</creatorcontrib><title>Intestinal ischemia and reperfusion injury in transgenic mice overexpressing copper-zinc superoxide dismutase</title><title>American Journal of Physiology: Cell Physiology</title><description>D. R. Deshmukh, O. Mirochnitchenko, V. S. Ghole, D. Agnese, P. C. Shah, M. Reddell, R. E. Brolin and M. Inouye
Department of Surgery, University of Medicine and Dentistry of New Jersey, New Brunswick 08903-0019, USA.
Superoxide dismutase (SOD) scavenges oxygen radicals that are implicated in
the pathogenesis of intestinal ischemia-reperfusion injury. The effect of
intestinal ischemia and reperfusion was investigated in transgenic mice
overexpressing human Cu-Zn SOD. Ischemia was induced by occluding the
superior mesenteric artery. Myeloperoxidase activity was determined as an
index of neutrophil infiltration, and malondialdehyde levels were measured
as an indicator of lipid peroxidation. Forty-five minutes of intestinal
ischemia followed by 4 h of reperfusion caused an increase in intestinal
levels of malondialdehyde in both nontransgenic and transgenic mice, but
the concentration of malondialdehyde was significantly greater in
nontransgenic mice. Intestinal ischemia-reperfusion also caused an increase
in intestinal and pulmonary myeloperoxidase activity in nontransgenic and
transgenic mice, but the transgenic mice had significantly lower levels of
myeloperoxidase activity than nontransgenic mice. Transgenic mice had
higher levels of intestinal SOD activity than nontransgenic mice. There
were no significant differences in the catalase or glutathione peroxidase
activities. In conclusion, our study demonstrates that the overexpression
of SOD protects tissues from neutrophil infiltration and lipid peroxidation
during intestinal ischemia-reperfusion.</description><issn>0363-6143</issn><issn>1522-1563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp10N1OwyAUwHFiNHNO34EXaC1QxvDOLH4sWeLNvCYMDhtLSxtodfPp7TI1euHVITn5kZM_QpgUOSGc3upda6CqciKlyKlgeZkbQlhxhsbDmmaET9k5GhdsyrIpKdklukppVxRFSadyhEaScSE4H6N6ETpInQ-6wj6ZLdReYx0sjtBCdH3yTcA-7Pp4GAbuog5pA8EbXHsDuHmDCPs2Qko-bLBp2kFlHz4YnPrh2ey9BWx9qvtOJ7hGF05XCW6-5gS9Pj6s5s_Z8uVpMb9fZqaUrMs0I5IS7uxagiuH8wnQtbGcGrnmjtNZKcBwAC2ZYK60QjumLVhKxZoRLtgEzU7_mtikFMGpNvpax4MihToGVF8B1TGgGgKqUs2PAQean-jWb7bvPoJqt4ehQtVsDj_qL7j7Hzz2VbWCffctf0HVWsc-ARhGjqg</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Deshmukh, Devendra R</creator><creator>Mirochnitchenko, Oleg</creator><creator>Ghole, Vikram S</creator><creator>Agnese, Doreen</creator><creator>Shah, Pritesh C</creator><creator>Reddell, Michael</creator><creator>Brolin, Robert E</creator><creator>Inouye, Masayori</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19971001</creationdate><title>Intestinal ischemia and reperfusion injury in transgenic mice overexpressing copper-zinc superoxide dismutase</title><author>Deshmukh, Devendra R ; Mirochnitchenko, Oleg ; Ghole, Vikram S ; Agnese, Doreen ; Shah, Pritesh C ; Reddell, Michael ; Brolin, Robert E ; Inouye, Masayori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-a319215fdb9ef41431e2bcd52c9b5f52847ec5eea9373f4d7af3aded227b31573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deshmukh, Devendra R</creatorcontrib><creatorcontrib>Mirochnitchenko, Oleg</creatorcontrib><creatorcontrib>Ghole, Vikram S</creatorcontrib><creatorcontrib>Agnese, Doreen</creatorcontrib><creatorcontrib>Shah, Pritesh C</creatorcontrib><creatorcontrib>Reddell, Michael</creatorcontrib><creatorcontrib>Brolin, Robert E</creatorcontrib><creatorcontrib>Inouye, Masayori</creatorcontrib><collection>CrossRef</collection><jtitle>American Journal of Physiology: Cell Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deshmukh, Devendra R</au><au>Mirochnitchenko, Oleg</au><au>Ghole, Vikram S</au><au>Agnese, Doreen</au><au>Shah, Pritesh C</au><au>Reddell, Michael</au><au>Brolin, Robert E</au><au>Inouye, Masayori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intestinal ischemia and reperfusion injury in transgenic mice overexpressing copper-zinc superoxide dismutase</atitle><jtitle>American Journal of Physiology: Cell Physiology</jtitle><date>1997-10-01</date><risdate>1997</risdate><volume>273</volume><issue>4</issue><spage>C1130</spage><epage>C1135</epage><pages>C1130-C1135</pages><issn>0363-6143</issn><eissn>1522-1563</eissn><abstract>D. R. Deshmukh, O. Mirochnitchenko, V. S. Ghole, D. Agnese, P. C. Shah, M. Reddell, R. E. Brolin and M. Inouye
Department of Surgery, University of Medicine and Dentistry of New Jersey, New Brunswick 08903-0019, USA.
Superoxide dismutase (SOD) scavenges oxygen radicals that are implicated in
the pathogenesis of intestinal ischemia-reperfusion injury. The effect of
intestinal ischemia and reperfusion was investigated in transgenic mice
overexpressing human Cu-Zn SOD. Ischemia was induced by occluding the
superior mesenteric artery. Myeloperoxidase activity was determined as an
index of neutrophil infiltration, and malondialdehyde levels were measured
as an indicator of lipid peroxidation. Forty-five minutes of intestinal
ischemia followed by 4 h of reperfusion caused an increase in intestinal
levels of malondialdehyde in both nontransgenic and transgenic mice, but
the concentration of malondialdehyde was significantly greater in
nontransgenic mice. Intestinal ischemia-reperfusion also caused an increase
in intestinal and pulmonary myeloperoxidase activity in nontransgenic and
transgenic mice, but the transgenic mice had significantly lower levels of
myeloperoxidase activity than nontransgenic mice. Transgenic mice had
higher levels of intestinal SOD activity than nontransgenic mice. There
were no significant differences in the catalase or glutathione peroxidase
activities. In conclusion, our study demonstrates that the overexpression
of SOD protects tissues from neutrophil infiltration and lipid peroxidation
during intestinal ischemia-reperfusion.</abstract><pmid>9357755</pmid><doi>10.1152/ajpcell.1997.273.4.c1130</doi></addata></record> |
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| source | American Physiological Society Free |
| title | Intestinal ischemia and reperfusion injury in transgenic mice overexpressing copper-zinc superoxide dismutase |
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