Exercise training increases lipid metabolism gene expression in human skeletal muscle

• Published in: American journal of physiology: endocrinology and metabolism 2002-07, Vol.283 (1), p.E66-E72
• Main Authors: Tunstall, Rebecca J, Mehan, Kate A, Wadley, Glenn D, Collier, Gregory R, Bonen, Arend, Hargreaves, Mark, Cameron-Smith, David
• Format: Article
• Language: English
• Subjects: 3-Hydroxyacyl CoA Dehydrogenases - genetics; 3-Hydroxyacyl CoA Dehydrogenases - metabolism; Adult; Carnitine O-Palmitoyltransferase - genetics; Carnitine O-Palmitoyltransferase - metabolism; Carrier Proteins - genetics; Carrier Proteins - metabolism; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; CD36 Antigens; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Exercise - physiology; Exercise Test; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Female; Gene Expression Regulation - physiology; Humans; Lipid Metabolism; Male; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Muscle, Skeletal - metabolism; Neoplasm Proteins; Organic Anion Transporters - genetics; Organic Anion Transporters - metabolism; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; RNA, Messenger - metabolism; Sterol Regulatory Element Binding Protein 1; Time Factors; Transcription Factors - genetics; Transcription Factors - metabolism; Tumor Suppressor Proteins
• ISSN: 0193-1849; 1522-1555
• DOI: 10.1152/ajpendo.00475.2001

1  School of Health Sciences, Deakin University, Burwood, Victoria 3125; 2  Metabolic Research Unit, School of Health Sciences, Deakin University, Geelong, Victoria 3217, Australia; 3  Department of Kinesiology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada The effects of a single bout of exercise and exercise training on the expression of genes necessary for the transport and -oxidation of fatty acids (FA), together with the gene expression of transcription factors implicated in the regulation of FA homeostasis were investigated. Seven human subjects (3 male, 4 female, 28.9   ± 3.1 yr of age, range 20-42 yr, body mass index 22.6 kg/m 2 , range 17-26 kg/m 2 ) underwent a 9-day exercise training program of 60 min cycling per day at 63% peak oxygen uptake ( O 2 peak ; 104 ± 14   W). On days 1  and 9  of the program, muscle biopsies were sampled from the vastus lateralis muscle at rest, at the completion of exercise, and again 3 h postexercise. Gene expression of key components of FA transport [FA translocase (FAT/CD36), plasma membrane-associated FA-binding protein], -oxidation [carntine palmitoyltransferase(CPT) I, -hydroxyacyl-CoA dehydrogenase] and transcriptional control [peroxisome proliferator-activated receptor (PPAR) , PPAR , PPAR coactivator 1, sterol regulatory element-binding protein-1c] were unaltered by exercise when measured at the completion and at 3   h postexercise. Training increased total lipid oxidation by 24% ( P