Thermal instability of rat muscle sarcoplasmic reticulum Ca2+-ATPase function

Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 To examine the thermal instability and the role of sulfhydryl (SH) oxidation on sarcoplasmic reticulum (SR) Ca 2+ -ATPase function, crude homogenates were prepared from the white portion of the gastrocnemius (WG) ad...

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Bibliographic Details
Published in:American journal of physiology: endocrinology and metabolism 2002-10, Vol.283 (4), p.E722
Main Authors: Schertzer, J. D, Green, H. J, Tupling, A. R
Format: Article
Language:English
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Summary:Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 To examine the thermal instability and the role of sulfhydryl (SH) oxidation on sarcoplasmic reticulum (SR) Ca 2+ -ATPase function, crude homogenates were prepared from the white portion of the gastrocnemius (WG) adult rat muscles ( n  = 9) and incubated in vitro for 60 min either at a normal resting body temperature (37°C) or at a temperature indicative of exercise-induced hyperthermia (41°C) with DTT and without DTT (CON). In general, treatment with DTT resulted in higher Ca 2+ -ATPase and Ca 2+ uptake values (nmol · mg protein 1 · min 1 , P  
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.00204.2002