Role of cytochrome P-450 4A in oxygen sensing and NO production in rat cremaster resistance arteries

1  Laboratory for Physiology, Institute for Cardiovascular Research, Vrije Universiteit, 1071 BT Amsterdam; and 2  Academic Medical Center, Department of Medical Physics, University of Amsterdam, 1100 DE Amsterdam, The Netherlands The role of arachidonic acid metabolism and nitric oxide (NO) in hypo...

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Published in:American journal of physiology. Heart and circulatory physiology 1999-10, Vol.277 (4), p.H1546-H1552
Main Authors: Kerkhof, Cornel J. M, Bakker, Erik N. T. P, Sipkema, Pieter
Format: Article
Language:English
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Summary:1  Laboratory for Physiology, Institute for Cardiovascular Research, Vrije Universiteit, 1071 BT Amsterdam; and 2  Academic Medical Center, Department of Medical Physics, University of Amsterdam, 1100 DE Amsterdam, The Netherlands The role of arachidonic acid metabolism and nitric oxide (NO) in hypoxia-induced changes of vascular tone was investigated in first-order cannulated rat cremaster muscle resistance arteries. Spontaneous tone reduced arterial diameter from 179 ± 2 µm (fully dilated) to 98 ± 3 µm under normoxia (P O 2  = 150 mmHg). Hypoxia (P O 2 5-10 mmHg) had no significant effect on arterial diameter under conditions of spontaneous tone. The effect of hypoxia was not changed after blockade of cyclooxygenase with indomethacin or after blockade of lipoxygenase with nordihydroguaiaretic acid. However, after partial blockade of cytochrome P -450 4A enzymes with 17-octadecynoic acid (17-ODYA), hypoxia increased the diameter by 65 ± 6 µm ( P  
ISSN:0363-6135
1522-1539
DOI:10.1152/ajpheart.1999.277.4.H1546