Effect of tumor necrosis factor-alpha , IL-1beta , and IL-6 on interstitial fluid pressure in rat skin

• Published in: American journal of physiology. Heart and circulatory physiology 1999-11, Vol.277 (5), p.H1857
• Main Authors: Nedrebo, Torbjorn, Berg, Ansgar, Reed, Rolf K
• Format: Article
• Language: English
• ISSN: 0363-6135; 1522-1539
• DOI: 10.1152/ajpheart.1999.277.5.H1857

Department of Physiology, University of Bergen, Bergen, Norway Interstitial fluid pressure (P if ) decreases in several experimental models of acute inflammation, enhancing edema formation. The present study was designed to determine the effect of tumor necrosis factor- (TNF- ), interleukin (IL)-6, and IL-1 as well as lipopolysaccharides (LPS) on P if in a model of gram-negative sepsis. P if was measured in the paw skin of anesthetized rats (pentobarbital sodium, 50 mg/kg ip) using micropipettes (3-7 µm) and servo-controlled counterpressure technique. Test substances were injected intra-arterially (ia), intravenously (iv), or subdermally (sd). After intra-arterial or intravenous administration, the test substances were circulated for 1 min before circulatory arrest was induced with an intravenous injection of KCl while the rats were under pentobarbital anesthesia. Circulatory arrest was induced to avoid edema formation, which would raise interstitial fluid volume to cause a more positive P if . Administration of 0.5 ml of LPS (5 mg/ml ia) lowered P if significantly from control values of 0.2 ± 0.3 to 2.0 ± 0.3 mmHg ( P