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Chronic intrauterine pulmonary hypertension decreases calcium-sensitive potassium channel mRNA expression
1 Division of Pediatric Pulmonology and Critical Care Medicine, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota 55455; and 2 Division of Pediatric Pulmonology, University of Colorado School of Medicine, Denver, Colorado 80218 Calcium-sensitive potassium (K...
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| Published in: | American journal of physiology. Lung cellular and molecular physiology 2000-11, Vol.279 (5), p.857-L862 |
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| cites | cdi_FETCH-LOGICAL-c397t-cb06fc350ad39a97c127e965209efb329362e1bd971c6fd5d0037ad5115674623 |
| container_end_page | L862 |
| container_issue | 5 |
| container_start_page | 857 |
| container_title | American journal of physiology. Lung cellular and molecular physiology |
| container_volume | 279 |
| creator | Cornfield, David N Resnik, Ernesto R Herron, Jean M Abman, Steven H |
| description | 1 Division of Pediatric Pulmonology and Critical Care
Medicine, Department of Pediatrics, University of Minnesota Medical
School, Minneapolis, Minnesota 55455; and 2 Division of
Pediatric Pulmonology, University of Colorado School of Medicine,
Denver, Colorado 80218
Calcium-sensitive potassium
(K Ca ) channels play a critical role in mediating perinatal
pulmonary vasodilation. Because infants with persistent pulmonary
hypertension of the newborn (PPHN) have blunted vasodilator responses
to birth-related stimuli, we hypothesized that lung K Ca
channel gene expression is decreased in PPHN. To test this hypothesis,
we measured K Ca channel gene expression in distal lung
homogenates from both fetal lambs with severe pulmonary hypertension
caused by prolonged compression of the ductus arteriosus and
age-matched, sham-operated animals (controls). After at least 9 days of
compression of the ductus arteriosus, fetal lambs were killed. To
determine lung K Ca channel mRNA levels, primers were designed against the known sequence of the K Ca channel and
used in semiquantitative RT-PCR, with lung 18S rRNA content as an
internal control. Compared to that in control lambs, lung
K Ca channel mRNA content in the PPHN group was reduced by
26 ± 6% ( P |
| doi_str_mv | 10.1152/ajplung.2000.279.5.l857 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_highw</sourceid><recordid>TN_cdi_highwire_physiology_ajplung_279_5_L857</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72361499</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-cb06fc350ad39a97c127e965209efb329362e1bd971c6fd5d0037ad5115674623</originalsourceid><addsrcrecordid>eNp1kMFu1DAURS0EoqXtL0BW7BKe7bGNl9WIAtKISlVZWx7nZeLKcYKd0M7f49EMGjasbPndc598CPlAoaFUsE_2aQpL3DUMABqmdCOa8FmoV-SyTFlNBaxelzusoAYJ4oK8y_mpZAWAfEsuKAXBgcEl8es-jdG7ysc52WXG5CNW0xKGMdq0r_r9hGnGmP0YqxZdQpsxV84G55ehzofJ7H8XZJxtzuWtcr2NEUM1PPy4rfBlSpgP9DV509mQ8eZ0XpGfd18e19_qzf3X7-vbTe24VnPttiA7xwXYlmurlaNMoZaCgcZuy5nmkiHdtlpRJ7tWtABc2VYULVKtJONX5OOxd0rjrwXzbAafHYZgI45LNopxSVdal6A6Bl0ac07YmSn5oXzaUDAHy-Zk2Rwsm2LZCLMplgv5_rRi2Q7YnrmT1hKoj4He7_pnn9BM_b5ICONuf279t1D_P3-3hPCIL_Nf8MyZqe34H2nPowk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72361499</pqid></control><display><type>article</type><title>Chronic intrauterine pulmonary hypertension decreases calcium-sensitive potassium channel mRNA expression</title><source>American Physiological Society Free</source><creator>Cornfield, David N ; Resnik, Ernesto R ; Herron, Jean M ; Abman, Steven H</creator><creatorcontrib>Cornfield, David N ; Resnik, Ernesto R ; Herron, Jean M ; Abman, Steven H</creatorcontrib><description>1 Division of Pediatric Pulmonology and Critical Care
Medicine, Department of Pediatrics, University of Minnesota Medical
School, Minneapolis, Minnesota 55455; and 2 Division of
Pediatric Pulmonology, University of Colorado School of Medicine,
Denver, Colorado 80218
Calcium-sensitive potassium
(K Ca ) channels play a critical role in mediating perinatal
pulmonary vasodilation. Because infants with persistent pulmonary
hypertension of the newborn (PPHN) have blunted vasodilator responses
to birth-related stimuli, we hypothesized that lung K Ca
channel gene expression is decreased in PPHN. To test this hypothesis,
we measured K Ca channel gene expression in distal lung
homogenates from both fetal lambs with severe pulmonary hypertension
caused by prolonged compression of the ductus arteriosus and
age-matched, sham-operated animals (controls). After at least 9 days of
compression of the ductus arteriosus, fetal lambs were killed. To
determine lung K Ca channel mRNA levels, primers were designed against the known sequence of the K Ca channel and
used in semiquantitative RT-PCR, with lung 18S rRNA content as an
internal control. Compared to that in control lambs, lung
K Ca channel mRNA content in the PPHN group was reduced by
26 ± 6% ( P < 0.02), whereas lung voltage-gated
K + 2.1 mRNA content was unchanged. We conclude that lung
K Ca channel mRNA expression is decreased in an ovine model
of PPHN. Decreased K Ca channel gene expression may
contribute to the abnormal pulmonary vascular reactivity associated
with PPHN.
fetus; oxygen sensing; messenger RNA; pulmonary
vasodilation</description><identifier>ISSN: 1040-0605</identifier><identifier>EISSN: 1522-1504</identifier><identifier>DOI: 10.1152/ajplung.2000.279.5.l857</identifier><identifier>PMID: 11053020</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Animals ; Animals, Newborn ; Delayed Rectifier Potassium Channels ; Female ; Humans ; Hypertension, Pulmonary - embryology ; Hypertension, Pulmonary - genetics ; Lung - embryology ; Molecular Sequence Data ; Potassium Channels - chemistry ; Potassium Channels - genetics ; Potassium Channels, Voltage-Gated ; Pregnancy ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Sequence Homology, Amino Acid ; Sheep</subject><ispartof>American journal of physiology. Lung cellular and molecular physiology, 2000-11, Vol.279 (5), p.857-L862</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-cb06fc350ad39a97c127e965209efb329362e1bd971c6fd5d0037ad5115674623</citedby><cites>FETCH-LOGICAL-c397t-cb06fc350ad39a97c127e965209efb329362e1bd971c6fd5d0037ad5115674623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11053020$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cornfield, David N</creatorcontrib><creatorcontrib>Resnik, Ernesto R</creatorcontrib><creatorcontrib>Herron, Jean M</creatorcontrib><creatorcontrib>Abman, Steven H</creatorcontrib><title>Chronic intrauterine pulmonary hypertension decreases calcium-sensitive potassium channel mRNA expression</title><title>American journal of physiology. Lung cellular and molecular physiology</title><addtitle>Am J Physiol Lung Cell Mol Physiol</addtitle><description>1 Division of Pediatric Pulmonology and Critical Care
Medicine, Department of Pediatrics, University of Minnesota Medical
School, Minneapolis, Minnesota 55455; and 2 Division of
Pediatric Pulmonology, University of Colorado School of Medicine,
Denver, Colorado 80218
Calcium-sensitive potassium
(K Ca ) channels play a critical role in mediating perinatal
pulmonary vasodilation. Because infants with persistent pulmonary
hypertension of the newborn (PPHN) have blunted vasodilator responses
to birth-related stimuli, we hypothesized that lung K Ca
channel gene expression is decreased in PPHN. To test this hypothesis,
we measured K Ca channel gene expression in distal lung
homogenates from both fetal lambs with severe pulmonary hypertension
caused by prolonged compression of the ductus arteriosus and
age-matched, sham-operated animals (controls). After at least 9 days of
compression of the ductus arteriosus, fetal lambs were killed. To
determine lung K Ca channel mRNA levels, primers were designed against the known sequence of the K Ca channel and
used in semiquantitative RT-PCR, with lung 18S rRNA content as an
internal control. Compared to that in control lambs, lung
K Ca channel mRNA content in the PPHN group was reduced by
26 ± 6% ( P < 0.02), whereas lung voltage-gated
K + 2.1 mRNA content was unchanged. We conclude that lung
K Ca channel mRNA expression is decreased in an ovine model
of PPHN. Decreased K Ca channel gene expression may
contribute to the abnormal pulmonary vascular reactivity associated
with PPHN.
fetus; oxygen sensing; messenger RNA; pulmonary
vasodilation</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Delayed Rectifier Potassium Channels</subject><subject>Female</subject><subject>Humans</subject><subject>Hypertension, Pulmonary - embryology</subject><subject>Hypertension, Pulmonary - genetics</subject><subject>Lung - embryology</subject><subject>Molecular Sequence Data</subject><subject>Potassium Channels - chemistry</subject><subject>Potassium Channels - genetics</subject><subject>Potassium Channels, Voltage-Gated</subject><subject>Pregnancy</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sheep</subject><issn>1040-0605</issn><issn>1522-1504</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kMFu1DAURS0EoqXtL0BW7BKe7bGNl9WIAtKISlVZWx7nZeLKcYKd0M7f49EMGjasbPndc598CPlAoaFUsE_2aQpL3DUMABqmdCOa8FmoV-SyTFlNBaxelzusoAYJ4oK8y_mpZAWAfEsuKAXBgcEl8es-jdG7ysc52WXG5CNW0xKGMdq0r_r9hGnGmP0YqxZdQpsxV84G55ehzofJ7H8XZJxtzuWtcr2NEUM1PPy4rfBlSpgP9DV509mQ8eZ0XpGfd18e19_qzf3X7-vbTe24VnPttiA7xwXYlmurlaNMoZaCgcZuy5nmkiHdtlpRJ7tWtABc2VYULVKtJONX5OOxd0rjrwXzbAafHYZgI45LNopxSVdal6A6Bl0ac07YmSn5oXzaUDAHy-Zk2Rwsm2LZCLMplgv5_rRi2Q7YnrmT1hKoj4He7_pnn9BM_b5ICONuf279t1D_P3-3hPCIL_Nf8MyZqe34H2nPowk</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Cornfield, David N</creator><creator>Resnik, Ernesto R</creator><creator>Herron, Jean M</creator><creator>Abman, Steven H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001101</creationdate><title>Chronic intrauterine pulmonary hypertension decreases calcium-sensitive potassium channel mRNA expression</title><author>Cornfield, David N ; Resnik, Ernesto R ; Herron, Jean M ; Abman, Steven H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-cb06fc350ad39a97c127e965209efb329362e1bd971c6fd5d0037ad5115674623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Delayed Rectifier Potassium Channels</topic><topic>Female</topic><topic>Humans</topic><topic>Hypertension, Pulmonary - embryology</topic><topic>Hypertension, Pulmonary - genetics</topic><topic>Lung - embryology</topic><topic>Molecular Sequence Data</topic><topic>Potassium Channels - chemistry</topic><topic>Potassium Channels - genetics</topic><topic>Potassium Channels, Voltage-Gated</topic><topic>Pregnancy</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sheep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cornfield, David N</creatorcontrib><creatorcontrib>Resnik, Ernesto R</creatorcontrib><creatorcontrib>Herron, Jean M</creatorcontrib><creatorcontrib>Abman, Steven H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Lung cellular and molecular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cornfield, David N</au><au>Resnik, Ernesto R</au><au>Herron, Jean M</au><au>Abman, Steven H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chronic intrauterine pulmonary hypertension decreases calcium-sensitive potassium channel mRNA expression</atitle><jtitle>American journal of physiology. Lung cellular and molecular physiology</jtitle><addtitle>Am J Physiol Lung Cell Mol Physiol</addtitle><date>2000-11-01</date><risdate>2000</risdate><volume>279</volume><issue>5</issue><spage>857</spage><epage>L862</epage><pages>857-L862</pages><issn>1040-0605</issn><eissn>1522-1504</eissn><abstract>1 Division of Pediatric Pulmonology and Critical Care
Medicine, Department of Pediatrics, University of Minnesota Medical
School, Minneapolis, Minnesota 55455; and 2 Division of
Pediatric Pulmonology, University of Colorado School of Medicine,
Denver, Colorado 80218
Calcium-sensitive potassium
(K Ca ) channels play a critical role in mediating perinatal
pulmonary vasodilation. Because infants with persistent pulmonary
hypertension of the newborn (PPHN) have blunted vasodilator responses
to birth-related stimuli, we hypothesized that lung K Ca
channel gene expression is decreased in PPHN. To test this hypothesis,
we measured K Ca channel gene expression in distal lung
homogenates from both fetal lambs with severe pulmonary hypertension
caused by prolonged compression of the ductus arteriosus and
age-matched, sham-operated animals (controls). After at least 9 days of
compression of the ductus arteriosus, fetal lambs were killed. To
determine lung K Ca channel mRNA levels, primers were designed against the known sequence of the K Ca channel and
used in semiquantitative RT-PCR, with lung 18S rRNA content as an
internal control. Compared to that in control lambs, lung
K Ca channel mRNA content in the PPHN group was reduced by
26 ± 6% ( P < 0.02), whereas lung voltage-gated
K + 2.1 mRNA content was unchanged. We conclude that lung
K Ca channel mRNA expression is decreased in an ovine model
of PPHN. Decreased K Ca channel gene expression may
contribute to the abnormal pulmonary vascular reactivity associated
with PPHN.
fetus; oxygen sensing; messenger RNA; pulmonary
vasodilation</abstract><cop>United States</cop><pmid>11053020</pmid><doi>10.1152/ajplung.2000.279.5.l857</doi></addata></record> |
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| identifier | ISSN: 1040-0605 |
| ispartof | American journal of physiology. Lung cellular and molecular physiology, 2000-11, Vol.279 (5), p.857-L862 |
| issn | 1040-0605 1522-1504 |
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| source | American Physiological Society Free |
| subjects | Amino Acid Sequence Animals Animals, Newborn Delayed Rectifier Potassium Channels Female Humans Hypertension, Pulmonary - embryology Hypertension, Pulmonary - genetics Lung - embryology Molecular Sequence Data Potassium Channels - chemistry Potassium Channels - genetics Potassium Channels, Voltage-Gated Pregnancy Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment Sequence Homology, Amino Acid Sheep |
| title | Chronic intrauterine pulmonary hypertension decreases calcium-sensitive potassium channel mRNA expression |
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