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Alterations in myocardial signal transduction due to aging and chronic dynamic exercise
Molecular Physiology and Exercise Biochemistry Laboratories, Department of Kinesiology, University of Colorado, Boulder, Colorado 80309-0354 Roth, David A., Cynthia D. White, Deborah A. Podolin, and Robert S. Mazzeo. Alterations in myocardial signal transduction due to aging and chronic dynamic exer...
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Published in: | Journal of applied physiology (1985) 1998-01, Vol.84 (1), p.177-184 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
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Summary: | Molecular Physiology and Exercise Biochemistry Laboratories,
Department of Kinesiology, University of Colorado, Boulder, Colorado
80309-0354
Roth, David A., Cynthia D. White, Deborah A. Podolin, and
Robert S. Mazzeo. Alterations in myocardial signal transduction due to aging and chronic dynamic exercise. J. Appl. Physiol.
84(1): 177-184, 1998. Normal aging without disease leads to
diminished chronotropic and inotropic responses to catecholamine
stimulation, resulting in depressed cardiac function with stress. The
purpose of this study was to determine molecular mechanisms for
decrements in adrenergic responsiveness of the left ventricle (LV) due
to aging and to study the effects of chronic dynamic exercise on signal
transduction. We measured -adrenergic receptor ( -AR) density,
adenylyl cyclase (AC) activity, and G-protein content and distribution
in LV from 66 male Fischer 344 rats from three age groups that were
either sedentary or treadmill trained (60 min/day, 5 days/wk, 10 wk at
75% of the maximal capacity). Final ages were 7 mo
(young), 15 mo (middle-age), and 25 mo (old). There was no significant
difference in -AR density among groups as a function of age or
training. AC production of adenosine 3 ,5 -cyclic monophosphate (cAMP)
with the use of five pharmacological stimulations revealed that old
sedentary myocardium had depressed basal, receptor-dependent, G-protein-dependent, and AC catalyst stimulation (30-43%)
compared with hearts from young and middle-age sedentary rats. Training did not alter AC activity in either middle-age or old groups but did
increase G-protein-dependent cAMP production in young myocardium (12-34%). Immunodetectable concentrations of stimulatory and
inhibitory G proteins (G s and G i , respectively)
showed 43% less total G s with similar G i
content in hearts from old sedentary compared with middle-age sedentary
rats. When compared with young sedentary animals, G i
content was 39 and 50% higher in middle-age sedentary and old
sedentary myocardium, respectively. With age, there was a significant
shift in the -subunit of G s distribution from cytosolic fractions of LV homogenates to membrane-bound fractions (8-12% redistribution in middle-age sedentary vs. old sedentary). The most
significant training effect was a decrease in G i content in
hearts from old trained rats (23%), which resulted in values comparable with young sedentary rats and reduced the
G i /G s ratio by 27% in old-rat LV. We report
that age-associated reductio |
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ISSN: | 8750-7587 1522-1601 |
DOI: | 10.1152/jappl.1998.84.1.177 |