cDNA microarray analysis reveals novel candidate genes expressed in human peripheral blood following exhaustive exercise

• Published in: Physiological genomics 2005-11, Vol.23 (3), p.287-294
• Main Authors: Zieker, Derek, Fehrenbach, Elvira, Dietzsch, Janko, Fliegner, Judith, Waidmann, Marc, Nieselt, Kay, Gebicke-Haerter, Peter, Spanagel, Rainer, Simon, Perikles, Niess, Andreas Michael, Northoff, Hinnak
• Format: Article
• Language: English
• Subjects: Adult; Antigens, CD - genetics; Blood Proteins - genetics; DNA Primers; DNA, Complementary - genetics; Exercise - physiology; Gene Expression Regulation; Glutathione Transferase - genetics; Humans; Interleukin 1 Receptor Antagonist Protein; Intracellular Signaling Peptides and Proteins; Male; Oligonucleotide Array Sequence Analysis; Protein-Serine-Threonine Kinases - genetics; RNA - genetics; Running; Sialoglycoproteins - genetics; Tetraspanin 28
• ISSN: 1094-8341; 1531-2267
• DOI: 10.1152/physiolgenomics.00096.2005

1 Department of Transfusions Medicine, University of Tuebingen 2 Center for Bioinformatics Tuebingen, Department of Information and Cognitive Sciences, University of Tuebingen 3 Department of Sports Medicine, Medical Clinic, University of Tuebingen 4 Central Institute for Mental Health, Department of Psychopharmacology, Mannheim, Germany It is generally accepted that exhausting endurance exercise exhibits strong effects on the immune system. Such effects have been attributed to changes in the cellular composition of peripheral blood as well as to changes in the expression of plausible candidate genes. The list of candidate genes is far from being complete, since this issue has not yet been investigated in a systematic way. In this study, we used a custom-made cDNA microarray focused on inflammation as a screening approach to study gene expression in eight one-half marathon runners before, immediately after, and 24 h after exercise. Significant differential gene expression was verified by quantitative real-time PCR. Linear regression analysis showed that microarray expression analysis of cell type-specific surface molecules reflects the observed individual cellular shifts in peripheral blood cells with high statistical significance. In line with the results of former studies, we observed an upregulation of mitogen-activated protein kinase-activated protein kinase-2 (MAPKAP-K2), L -selectin, and IL-1 receptor antagonist (IL-1ra) after exhaustive exercise. The main results of this study report, for the first time, the downregulation of CD81; the upregulation of thioredoxin, which may play an important part in anti-oxidative defense; and, surprisingly, the downregulation of the anti-carcinogenic gene glutathione- S -transferase-3 (GSTM3) in peripheral blood. The study shows cDNA microarray expression analysis as a reliable systematic instrument to complete the list of candidate genes that may play a role in exhaustive exercise-induced modulation of the immune response. thioredoxin; CD81; oxidative; marathon