Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population

• Published in: Croatian medical journal 2007-08, Vol.48 (4.), p.473
• Main Authors: Projic, Petar, Škaro, Vedrana, Šamija, Ivana, Durmić-Pašić, Adaleta, Pojskić, Naris, Kovačević, Lejla, Bakal, Narcisa, Primorac, Dragan, Marjanović, Damir
• Format: Article
• Language: English
• Subjects: Croatian population; Identifiler; STR population data
• ISSN: 0353-9504; 1332-8166

Aim To study the distribution of allele frequencies of 15 short tandem repeat (STR) loci in a representative sample of Croatian population. Methods A total of 195 unrelated Caucasian individuals born in Croatia, from 14 counties and the City of Zagreb, were sampled for the analysis. All the tested individuals were voluntary donors. Buccal swab was used as the DNA source. AmpFlSTR® Identifiler® was applied to simultaneously amplify 15 STR loci. Total reaction volume was 12.5 μL. The PCR amplification was carried out in PE Gene Amp PCR System Thermal Cycler. Electrophoresis of the amplification products was preformed on an ABI PRISM 3130 Genetic Analyzer. After PCR amplification and separation by electrophoresis, raw data were compiled, analyzed, and numerical allele designations of the profiles were obtained. Deviation from Hardy-Weinberg equilibrium, observed and expected heterozygosity, power of discrimination, and power of exclusion were calculated. Bonferroni’s correction was used before each comparative analysis. Results We compared Croatian data with those obtained from geographically neighboring European populations. The significant difference (at P