Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

Ultrasound-assisted extraction of phenolic antioxidants from Lotus corniculatus was optimized using response surface methodology. The extraction was performed according to the Box-Behnken design with ethanol concentration, temperature, and pH, as independent variables. The responses were extraction...

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Bibliographic Details
Published in:Croatica Chemica Acta 2019, Vol.92 (3), p.1-377
Main Authors: Fumic, Barbara, Jug, Mario, Koncic, Marijana Zovko
Format: Article
Language:English
Subjects:
Acids
Analysis
Antioxidants
Antioxidants (Nutrients)
Calibration
Cardiovascular disease
Derivatives
Ethanol
Flavonoids
Food
genistein
Independent variables
Isoflavones
Job stress
kaempferol
Lotus corniculatus
Optimization
Phenolic acids
Phenols
Phenols (Class of compounds)
Phytochemicals
quercetin
radical scavenging activity
Response surface methodology
Scavenging
Solvents
Type 2 diabetes
Ultrasonic imaging
Ultrasound
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Summary:Ultrasound-assisted extraction of phenolic antioxidants from Lotus corniculatus was optimized using response surface methodology. The extraction was performed according to the Box-Behnken design with ethanol concentration, temperature, and pH, as independent variables. The responses were extraction yield, DPPH radical scavenging activity (RSA) [IC.sub.50] and content of different phenolic compounds (total phenols, flavonoids and phenolic acids, as well as quercetin, kaempferol and genistein derivatives). The models were used to calculate best conditions for maximal extraction of phenolic compounds and antiradical activity. Use of the optimized extraction parameters increased the content of quercetin and kaempferol derivatives more than tenfold (from 6.07 to 65.10 mg m[L.sup.-1] and 6.69 to 92.75 mg m[L.sup.-1], respectively). The results of this work stress the importance of careful selection of conditions for flavonoids extraction. Abundance of bioactive phenolics in L corniculatus extracts obtained under optimized extraction conditions opens the possibility for wider utilization of this plant. Keywords: genistein, kaempferol, Lotus corniculatus, quercetin, radical scavenging activity, response surface methodology.
ISSN:0011-1643
1334-417X
DOI:10.5562/cca3549