First classical and molecular cytogenetic analyses of Sperata acicularis (Siluriformes, Bagridae)

The first chromosomal analysis of Salween shovelhead catfish (Sperata acicularis) was undertaken by classical cytogenetic and fluorescence in situ hybridization (FISH) techniques in the present study. Ten male and ten female fish were obtained from Salween River, Mae Hong Son Province, Northern Thai...

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Published in:TAIWANIA 2021-05, Vol.66 (2), p.203-213
Main Authors: Sukhonthip DITCHAROEN, Sudarat KHENSUWAN, Kriengkrai SEETAPAN, Phongphan SOONTHORNVIPAT, Chatmongkon SUWANNAPOOM, Krit PINTHONG, Sampan TONGNUNUI, Marcelo de Bello CIOFFI, Thomas LIEHR, Alongklod TANOMTONG, Weerayuth SUPIWONG
Format: Article
Language:Chinese
Subjects:
Chromosome
FISH
karyotype
Nucleolar Organizer Regions
SCIE
Scopus
Sperata acicularis
TSCI
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Summary:The first chromosomal analysis of Salween shovelhead catfish (Sperata acicularis) was undertaken by classical cytogenetic and fluorescence in situ hybridization (FISH) techniques in the present study. Ten male and ten female fish were obtained from Salween River, Mae Hong Son Province, Northern Thailand. The mitotic chromosome preparation was directly performed from kidney tissues. Conventional Giemsa staining, Ag-NOR staining, and molecular cytogenetics techniques with FISH using 5S, 18S rDNAs, and microsatellites d(CA)15 and d(GC)15 repeats as probes were conducted. The results indicated that the diploid chromosome number of S. acicularis was 2n = 56. The fundamental number (NF) was 110 both for males and females. The karyotype is composed of 18 large metacentric, 10 large submetacentric, 14 medium metacentric, 12 medium submetacentric and 2 medium acrocentric chromosomes; sex chromosomes could not be identified. NORs localized at the subtelomeric region of the short arm of metacentric chromosome pair 3, wh
ISSN:0372-333X