3D reconstruction of 2D crystals from projections in real space

A new algorithm for 3D reconstruction of two-dimensional crystals from projections is presented, and applied to biological macromolecules imaged using electron microscopy. Its main departure from the traditional approach is that it works in real space, rather than in Fourier space, and is iterative....

Full description

Saved in:
Bibliographic Details
Main Authors: Marabini, R., Herman, G.T., Sorzano, C.O.S., Carazo, J.M., Fernandez, J.J., Matej, S.
Format: Conference Proceeding
Language:English
Subjects:
Crystallization
Crystals
Electron microscopy
Fourier transforms
Image reconstruction
Iterative algorithms
Iterative methods
Lattices
Molecular biophysics
Transmission electron microscopy
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new algorithm for 3D reconstruction of two-dimensional crystals from projections is presented, and applied to biological macromolecules imaged using electron microscopy. Its main departure from the traditional approach is that it works in real space, rather than in Fourier space, and is iterative. This approach has the advantage of making it convenient to introduce additional constraints (such as the support of the function to be reconstructed, which may be known from alternative measurements) and has the potential of more accurately modeling the electron microscope image formation process.
DOI:10.1109/ISBI.2002.1029351