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Characterization of an in vitro model of intestinal cells as a test-bed for mCIPO therapies

Chronic Intestinal Pseudo-Obstruction in children (CIPO) comprises a group of ultra-rare and debilitating diseases characterized by severe motility defects affecting the entire digestive tract. With limited effective therapies, particularly for myopathic CIPO, we aim at characterizing the behavior o...

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Main Authors:	Corsello, Lorenzo, Marra, Simona, Saavedra, Adriana, Picco, Cristiana, Parodi, Giulia, Falleroni, Fabio, Gandullia, Paolo, Viti, Federica, Chiappalone, Michela
Format:	Conference Proceeding
Language:	English
Subjects:	Biological system modeling Chemicals CIPO contraction Diseases Electrical stimulation electroceutical Electrodes Engineering in medicine and biology Human Intestinal Smooth Muscle Cells In vitro Intestines Micro Electrode Arrays Muscles
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creator	Corsello, Lorenzo Marra, Simona Saavedra, Adriana Picco, Cristiana Parodi, Giulia Falleroni, Fabio Gandullia, Paolo Viti, Federica Chiappalone, Michela
description	Chronic Intestinal Pseudo-Obstruction in children (CIPO) comprises a group of ultra-rare and debilitating diseases characterized by severe motility defects affecting the entire digestive tract. With limited effective therapies, particularly for myopathic CIPO, we aim at characterizing the behavior of an intestinal smooth muscle cell line undergoing a set of chemical and physical stimulations, to assess their effect. To this end, we adopted healthy Human Intestinal Smooth Muscle Cells, plated on standard coverslips and, for the first time, Micro Electrode Arrays (MEAs). After obtaining stable cultures, we performed pharmacological and electrical stimulation, evaluating the contraction level as readout. We found an increase in the contraction levels ascribable to stimulation respect to basal condition. Our results can be considered introductory for new therapies for treating intestine motility disorders.
doi_str_mv	10.1109/EMBC53108.2024.10782258
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subjects	Biological system modeling Chemicals CIPO contraction Diseases Electrical stimulation electroceutical Electrodes Engineering in medicine and biology Human Intestinal Smooth Muscle Cells In vitro Intestines Micro Electrode Arrays Muscles
title	Characterization of an in vitro model of intestinal cells as a test-bed for mCIPO therapies
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