All optical histology of brain tissue: serial ablation and multiphoton imaging with femtosecond laser pulses

We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.

Saved in:
Bibliographic Details
Main Authors: Tsai, P.S., Friedman, B., Lev-Ram, V., Xiong, Q., Tsien, R.Y., Kleinfeld, D., Ifarraguerri, A.I., Thompson, B.D., Squier, J.A.
Format: Conference Proceeding
Language:English
Subjects:
Brain
Fluorescence
Laser ablation
Optical imaging
Optical pulses
Optical scattering
Proteins
Rough surfaces
Surface roughness
Ultrafast optics
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.