Selective two-photon imaging with shaped femtosecond pulses

Selective two-photon microscopy of fluorescent probe molecules using phase-modulated ultrashort laser pulses is demonstrated on fluorescent probes used for biological imaging. Maximum contrast in the excitation of these molecules is achieved using multiphoton intrapulse interference.

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Bibliographic Details
Main Authors: Pastirk, I., Dela Cruz, J.M., Lozovoy, V.V., Dantus, M.
Format: Conference Proceeding
Language:English
Subjects:
Absorption
Fluorescence
Interference
Laser excitation
Microscopy
Optical pulse shaping
Phase modulation
Probes
Pulse modulation
Pulse shaping methods
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Description
Summary:Selective two-photon microscopy of fluorescent probe molecules using phase-modulated ultrashort laser pulses is demonstrated on fluorescent probes used for biological imaging. Maximum contrast in the excitation of these molecules is achieved using multiphoton intrapulse interference.