Comparison of impedance at the microelectrode-saline and microelectrode-culture medium interface

The impedance characteristics of gold-plated indium-tin-oxide microelectrodes immersed in culture medium (MEM) are described and compared with the impedance characteristics observed when those microelectrodes are immersed in isotonic saline. For microelectrode areas of approximately 100 mu m/sup 2/,...

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Bibliographic Details
Published in:IEEE transactions on biomedical engineering 1992-11, Vol.39 (11), p.1123-1129
Main Authors: Carter, S.J., Linker, C.J., Turkle-Huslig, T., Howard, L.L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Capacitance
Culture Media - chemistry
Current density
Electric Impedance
Electrochemistry
Electrodes
Electrophysiology
Frequency
Fundamental and applied biological sciences. Psychology
Gold - chemistry
Immune system
Impedance
In vitro
Isotonic Solutions
Microelectrodes
Molecular biophysics
Physical chemistry in biology
Sodium Chloride - chemistry
Voltage
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Description
Summary:The impedance characteristics of gold-plated indium-tin-oxide microelectrodes immersed in culture medium (MEM) are described and compared with the impedance characteristics observed when those microelectrodes are immersed in isotonic saline. For microelectrode areas of approximately 100 mu m/sup 2/, applied voltage levels of 5, 50 and 100 mV, and for frequencies of from 100 Hz to 10 kHz the resistance, capacitance, capacitive reactance, and total impedance are given as a function of frequency both in culture medium and in saline. The results, which hold for current densities ranging from 0.45 to 700 pA/ mu /sup 2/, are compared. Also given are the alpha and K values determining the frequency characteristics of the interface resistance and capacitance in medium and in saline.< >
ISSN:0018-9294
1558-2531
DOI:10.1109/10.168691