Nanotube Molecular Probes: DNA Hybridization using Single Walled Carbon Nanotubes as Biomarkers

Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequ...

Full description

Saved in:
Bibliographic Details
Main Authors: Chengfan Cao, Sanghyun Hong, Jae-Boong Choi, Young-Jin Kim, Seunghyun Baik, Eung-Soo Hwang, Hye-Jin Jung, Chang-Yong Cha
Format: Conference Proceeding
Language:English
Subjects:
Bioinformatics
Biomarkers
Biomedical optical imaging
Carbon nanotubes
DNA
Genomics
Optical sensors
Photobleaching
Probes
Sequences
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs' possibility of being generic nano-bio markers for precise detection of specific kinds of genes.
ISSN:1930-0395
2168-9229
DOI:10.1109/ICSENS.2007.355861