Development of in situ ATP quantitative analysis system "IISA-ATP"

An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified...

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Bibliographic Details
Main Authors: Fukuzawa, N., Fukuba, T., Fujii, T.
Format: Conference Proceeding
Language:English
Subjects:
ATP
Biological materials
Calibration
Ecosystems
Extracellular
IISA-ATP
in-situ analysis
Luciferin-Luciferase reaction
Microfluidics
Optical detectors
Optical materials
PDMS
Pollution measurement
Prototypes
Sea measurements
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Summary:An integrated in situ analyzer for ATP concentration (IISA-ATP) has been developed in this study. Concept of IISA-ATP that has function for cell lysis, extracellular ATP elimination, ATP quantification with luciferin-luciferase reaction and in situ calibration is proposed in this study. A simplified prototype of PDMS based microfluidic chip that has a coiled microchannel pattern for optical detection is developed with micro fabrication technique. As a result of evaluation of the prototype, more than 10 -10 M range of ATP was successfully detected
DOI:10.1109/MMB.2006.251544