Assembly of Single Cells Array using Image Dielectrophoresis

Cell arraying with single cell per trap is demonstrated using image dielectrophoresis(DEP) and leads to a 5 by 5 single cells array. The tunable energy wells, which were induced by established patterns and image control, were used to confine NIH/3T3 cells. Image was used to help cells overcome the e...

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Bibliographic Details
Main Authors: Lu, Y.S., Liang, Y.L., Huang, Y.P., Huang, J.Y., Lee, C., Yeh, J.A.
Format: Conference Proceeding
Language:English
Subjects:
Amorphous silicon
Assembly
Biological cells
Biomedical monitoring
Cell arraying
Cells (biology)
DEP
Dielectrophoresis
Energy barrier
Image dielectrophoresis
Microscopy
Single cells array
Sugar
Temperature
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Summary:Cell arraying with single cell per trap is demonstrated using image dielectrophoresis(DEP) and leads to a 5 by 5 single cells array. The tunable energy wells, which were induced by established patterns and image control, were used to confine NIH/3T3 cells. Image was used to help cells overcome the energy barriers. It allows the controllability to rearrange cells, including locally dislodging the excess cells and repositioning them in non-occupant traps. The viability of post-assembled cells that were identified by fluorescence staining of FDA is about 90%. The continuous temperature monitoring shows that the device used reached its thermal equilibrium of 36degC, close to the 3T3 culture temperature of 37degC.
ISSN:2159-547X
DOI:10.1109/SENSOR.2007.4300170