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Assembly of Single Cells Array using Image Dielectrophoresis
Cell arraying with single cell per trap is demonstrated using image dielectrophoresis(DEP) and leads to a 5 by 5 single cells array. The tunable energy wells, which were induced by established patterns and image control, were used to confine NIH/3T3 cells. Image was used to help cells overcome the e...
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| creator | Lu, Y.S. Liang, Y.L. Huang, Y.P. Huang, J.Y. Lee, C. Yeh, J.A. |
| description | Cell arraying with single cell per trap is demonstrated using image dielectrophoresis(DEP) and leads to a 5 by 5 single cells array. The tunable energy wells, which were induced by established patterns and image control, were used to confine NIH/3T3 cells. Image was used to help cells overcome the energy barriers. It allows the controllability to rearrange cells, including locally dislodging the excess cells and repositioning them in non-occupant traps. The viability of post-assembled cells that were identified by fluorescence staining of FDA is about 90%. The continuous temperature monitoring shows that the device used reached its thermal equilibrium of 36degC, close to the 3T3 culture temperature of 37degC. |
| doi_str_mv | 10.1109/SENSOR.2007.4300170 |
| format | conference_proceeding |
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The continuous temperature monitoring shows that the device used reached its thermal equilibrium of 36degC, close to the 3T3 culture temperature of 37degC.</description><subject>Amorphous silicon</subject><subject>Assembly</subject><subject>Biological cells</subject><subject>Biomedical monitoring</subject><subject>Cell arraying</subject><subject>Cells (biology)</subject><subject>DEP</subject><subject>Dielectrophoresis</subject><subject>Energy barrier</subject><subject>Image dielectrophoresis</subject><subject>Microscopy</subject><subject>Single cells array</subject><subject>Sugar</subject><subject>Temperature</subject><issn>2159-547X</issn><isbn>9781424408412</isbn><isbn>1424408415</isbn><isbn>1424408423</isbn><isbn>9781424408429</isbn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2007</creationdate><recordtype>conference_proceeding</recordtype><sourceid>6IE</sourceid><recordid>eNo1j1FLwzAUhSMqOGd_wV7yB1pz06Q3AV9K3XQwHFgF30aW3s5KZ0cyH_rvHTjPy-E7Dx8cxmYgMgBh7-v5S71-zaQQmKlcCEBxwW5BSaWEUTK_ZIlF888gr9hEgrapVvhxw5IYv8QpuS20xQl7KGOk_bYf-dDyuvve9cQr6vvIyxDcyH_iaePLvdsRf-yoJ38Mw-FzCBS7eMeuW9dHSs49Ze-L-Vv1nK7WT8uqXKUdoD6mDZDTomix0Mo7SeC1AdLQeItk0YtWGeOtlaSs2Xovc0KJaFzrVQMN5lM2-_N2RLQ5hG7vwrg5n89_ARrHS4A</recordid><startdate>200706</startdate><enddate>200706</enddate><creator>Lu, Y.S.</creator><creator>Liang, Y.L.</creator><creator>Huang, Y.P.</creator><creator>Huang, J.Y.</creator><creator>Lee, C.</creator><creator>Yeh, J.A.</creator><general>IEEE</general><scope>6IE</scope><scope>6IL</scope><scope>CBEJK</scope><scope>RIE</scope><scope>RIL</scope></search><sort><creationdate>200706</creationdate><title>Assembly of Single Cells Array using Image Dielectrophoresis</title><author>Lu, Y.S. ; Liang, Y.L. ; Huang, Y.P. ; Huang, J.Y. ; Lee, C. ; Yeh, J.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i175t-d1ea506f7654ca2e1c581e51dc97e97c0f488c992e498bcc23e72778afc4d1d73</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Amorphous silicon</topic><topic>Assembly</topic><topic>Biological cells</topic><topic>Biomedical monitoring</topic><topic>Cell arraying</topic><topic>Cells (biology)</topic><topic>DEP</topic><topic>Dielectrophoresis</topic><topic>Energy barrier</topic><topic>Image dielectrophoresis</topic><topic>Microscopy</topic><topic>Single cells array</topic><topic>Sugar</topic><topic>Temperature</topic><toplevel>online_resources</toplevel><creatorcontrib>Lu, Y.S.</creatorcontrib><creatorcontrib>Liang, Y.L.</creatorcontrib><creatorcontrib>Huang, Y.P.</creatorcontrib><creatorcontrib>Huang, J.Y.</creatorcontrib><creatorcontrib>Lee, C.</creatorcontrib><creatorcontrib>Yeh, J.A.</creatorcontrib><collection>IEEE Electronic Library (IEL) Conference Proceedings</collection><collection>IEEE Proceedings Order Plan All Online (POP All Online) 1998-present by volume</collection><collection>IEEE Xplore All Conference Proceedings</collection><collection>IEEE/IET Electronic Library</collection><collection>IEEE Proceedings Order Plans (POP All) 1998-Present</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Lu, Y.S.</au><au>Liang, Y.L.</au><au>Huang, Y.P.</au><au>Huang, J.Y.</au><au>Lee, C.</au><au>Yeh, J.A.</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Assembly of Single Cells Array using Image Dielectrophoresis</atitle><btitle>TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference</btitle><stitle>SENSOR</stitle><date>2007-06</date><risdate>2007</risdate><spage>473</spage><epage>476</epage><pages>473-476</pages><issn>2159-547X</issn><isbn>9781424408412</isbn><isbn>1424408415</isbn><eisbn>1424408423</eisbn><eisbn>9781424408429</eisbn><abstract>Cell arraying with single cell per trap is demonstrated using image dielectrophoresis(DEP) and leads to a 5 by 5 single cells array. The tunable energy wells, which were induced by established patterns and image control, were used to confine NIH/3T3 cells. Image was used to help cells overcome the energy barriers. It allows the controllability to rearrange cells, including locally dislodging the excess cells and repositioning them in non-occupant traps. The viability of post-assembled cells that were identified by fluorescence staining of FDA is about 90%. The continuous temperature monitoring shows that the device used reached its thermal equilibrium of 36degC, close to the 3T3 culture temperature of 37degC.</abstract><pub>IEEE</pub><doi>10.1109/SENSOR.2007.4300170</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 2159-547X |
| ispartof | TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference, 2007, p.473-476 |
| issn | 2159-547X |
| language | eng |
| recordid | cdi_ieee_primary_4300170 |
| source | IEEE Electronic Library (IEL) Conference Proceedings |
| subjects | Amorphous silicon Assembly Biological cells Biomedical monitoring Cell arraying Cells (biology) DEP Dielectrophoresis Energy barrier Image dielectrophoresis Microscopy Single cells array Sugar Temperature |
| title | Assembly of Single Cells Array using Image Dielectrophoresis |
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