High Sensitivity Micropillar Electrosprayionization Chip Fabricated of Silicon

We have designed, fabricated and tested a micropillar array electrospray ionization (μPESI) chip for mass spectrometer (MS) analysis of drugs and biomolecules. The chip has a lidless microstructured channel that contains a perfectly ordered array of micropillars. The typical sample volume is 2.5 μl...

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Bibliographic Details
Main Authors: Sainiemi, L., Nissila, T., Sikanen, T., Kotiaho, T., Kostiainen, R., Ketola, R.A., Franssila, S.
Format: Conference Proceeding
Language:English
Subjects:
Drugs
Dynamic range
electrospray ionization
Ionization
Linearity
mass spectrometry
Mass spectroscopy
micropillars
Molecular biophysics
Semiconductor device measurement
Silicon
silicon microfabrication
Stability
Testing
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Description
Summary:We have designed, fabricated and tested a micropillar array electrospray ionization (μPESI) chip for mass spectrometer (MS) analysis of drugs and biomolecules. The chip has a lidless microstructured channel that contains a perfectly ordered array of micropillars. The typical sample volume is 2.5 μl and the sample flow in the channel is spontaneous. The limit of detection for verapamil measured with MS/MS was 30 pmol/1. The system shows also quantitative linearity (r 2 =0.997) with linear dynamic range of at least six orders of magnitude and good stability (standard deviation < 4%) at measurement for 60 minutes.
ISSN:2159-547X
DOI:10.1109/SENSOR.2007.4300501