Miniaturized Sensor for Microbial Pathogens DNA and Chemical Toxins

A miniaturized, inexpensive and battery operated ultra-sensitive nanomaterial-based fluorescence resonance energy transfer (NSET) probe has been developed and evaluated for screening microbial pathogens DNA and chemical toxins at high sensitivity and selectivity. This paper illustrates application o...

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Bibliographic Details
Published in:IEEE sensors journal 2008-06, Vol.8 (6), p.693-700
Main Authors: Darbha, G.K., Eumin Lee, Anderson, Y.R., Fowler, P., Mitchell, K., Ray, P.C.
Format: Article
Language:English
Subjects:
Batteries
Campylobacter
Chemical analysis
Chemical sensors
Clostridium perfringens
DNA
Energy exchange
Fluorescence
fluorescence resonance energy transfer
Gold
gold nanoparticles
mercury
Pathogens
Probes
Resonance
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Summary:A miniaturized, inexpensive and battery operated ultra-sensitive nanomaterial-based fluorescence resonance energy transfer (NSET) probe has been developed and evaluated for screening microbial pathogens DNA and chemical toxins at high sensitivity and selectivity. This paper illustrates application of the NSET portable device to detect microbial pathogens, e.g., Campylobacter, C. perfringens, and S. aureus DNA based on quenching of the NSET signal by gold nanoparticles to detect single base-mismatch DNA with high sensitivity (600 fM). Presence of the fluorescence signal indicates that the target DNA has been detected. We also report that the NSET probe is capable of screening chemical toxins like mercury from contaminated water with excellent sensitivity (2 parts-per-trillion) and selectivity for over competing analytes.
ISSN:1530-437X
1558-1748
DOI:10.1109/JSEN.2008.922727