A Fast 3D Volume Reconstruction for Confocal Micro-rotation Cell Imaging

We propose a fast reconstruction algorithm for nonadherent living cell images from micro-rotational confocal microscopy. This procedure is called bi-protocol. Its key point consists in coupling micro-rotation data and conventional z-stack. Doing so, the estimation of the micro-rotation slice positio...

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Bibliographic Details
Main Authors: Yong Yu, Trouve, A., Chalmond, B.
Format: Conference Proceeding
Language:English
Subjects:
Biomedical engineering
Biomedical imaging
Biomedical informatics
Biomedical optical imaging
Image reconstruction
Interpolation
Optical imaging
Optical microscopy
Reconstruction algorithms
Yield estimation
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Description
Summary:We propose a fast reconstruction algorithm for nonadherent living cell images from micro-rotational confocal microscopy. This procedure is called bi-protocol. Its key point consists in coupling micro-rotation data and conventional z-stack. Doing so, the estimation of the micro-rotation slice positions becomes a slice-to-volume registration allowing to reduce drastically the computing time. The validation of our procedure is performed on real data which gives significant improvement compared with the conventional z-stacking imaging.
ISSN:1948-2914
1948-2922
DOI:10.1109/BMEI.2008.201