Atomic Force Microscope Observation on CHO Cell Membrane after Electromagnetic Pulses Irradiation

Atomic force microscope (AFM) has been used to visualize the morphological change on the surface of CHO cell membrane before and after Electromagnetic pulses (EMP)irradiation. The results showed that there were different sizes and shapes of membrane perforate (the width is 0.66-0.39 um, the depth is...

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Bibliographic Details
Main Authors: Zeng Li-Hua, Li Yuan, Miao Xia, Wang Xiao-Wu, Lang Hai-Yang, Zhang Jie, Li Yu-Rong, Ren Dong-Qing, Guo Guo-Zhen
Format: Conference Proceeding
Language:English
Subjects:
Atomic force microscope
Atomic force microscopy
Biomembranes
cell membrane
Cells (biology)
CHO cell
Electromagnetic forces
electromagnetic pulse (EMP)
EMP radiation effects
Pulse shaping methods
Shape
Space vector pulse width modulation
Surface morphology
Visualization
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Summary:Atomic force microscope (AFM) has been used to visualize the morphological change on the surface of CHO cell membrane before and after Electromagnetic pulses (EMP)irradiation. The results showed that there were different sizes and shapes of membrane perforate (the width is 0.66-0.39 um, the depth is 392.95 nm) after 400 kV/m 200 pulses EMP irradiation. The numbers of cell membrane perforates were decreased with EMP field intensity and the pulses numbers decreasing. It is suggested that EMP irradiation can cause the membrane perforate of CHO cell directly. The numbers of cell membrane perforates were increased with EMP field intensity and the pulses numbers increasing. EMP induced membrane perforate may play a very important role in EMP bio-effects.
DOI:10.1109/ISAPE.2008.4735401