Bead-Immobilized Molecular Beacons for High Throughput SNP Genotyping Via a Microfluidic System

This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trappin...

Full description

Saved in:
Bibliographic Details
Main Authors: Sochol, R.D., Mahajerin, A., Casavant, B.P., Singh, P., Dueck, M., Lee, L.P., Lin, L.
Format: Conference Proceeding
Language:English
Subjects:
Bioinformatics
DNA
Genomics
Humans
Liver diseases
Microfluidics
Probes
Sequences
Temperature sensors
Throughput
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (muPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.
ISSN:1084-6999
DOI:10.1109/MEMSYS.2009.4805379