Novel Optical Nanobiosensor Based on Localized Surface Plasmon Resonance Assembled with Gold Colloid Nanoparticles

A novel optical biosensor assembled with gold colloidal nanoparticles on gold plate surface has been constructed via 1,4-dithiothreitol and L-cysteic acid for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface p...

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Bibliographic Details
Main Authors: Ling Zhang, Zhong Cao, Xun Li, Ming Tu, Meng-Xue Zeng, Xi-Xi Huang, Su-Li Guo, Mei-Li Chi, Chuan-Qi Chen, Gui-Fu Ma, Yu Cao, Ning Gu
Format: Conference Proceeding
Language:English
Subjects:
Assembly
Biomedical optical imaging
Biosensors
DNA
Gold
Nanoparticles
Optical surface waves
Plasmons
Proteins
Resonance
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Summary:A novel optical biosensor assembled with gold colloidal nanoparticles on gold plate surface has been constructed via 1,4-dithiothreitol and L-cysteic acid for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The prepared nano gold sol were characterized by using UV-Vis absorption spectroscopy, transmission electron microscopy (TEM), and cyclic voltammetry (CV), indicating that the Au colloidals were sphere-shaped and narrow-dispersed size distribution with a diameter of 24 plusmn 0.2 nm. It caused 2.80 nm of red shift in the peak wavelength (lambda max ) of LSPR spectrum for the nanosensor exposed to c-Myc (3C7) mouse monoclonal IgG and 3.36 nm of red shift to the corresponding antibody by the immuno-reaction subsequently. The LSPR nanobiosensor has been successfully applied to recognition of a DNA plasmid of pCMV-Myc Mammalian Expression Vector.
ISSN:2151-7614
2151-7622
DOI:10.1109/ICBBE.2009.5163124