3D fluorescent particle tracking with nanometer scale accuracies using a double-helix point spread function

We demonstrate parallel three-dimensional (3D) tracking of multiple fluorescent microspheres with nanometer scale accuracies by engineering the 3D point spread function of a wide-field microscope to present a double-helix along the optical axis.

Saved in:
Bibliographic Details
Main Authors: Pavani, S.R.P., Piestun, R.
Format: Conference Proceeding
Language:English
Subjects:
(110.1758) Computational imaging
(180.6900) Three-dimensional microscopy
(280.7250) Velocimetry
Biomedical optical imaging
Fluorescence
Focusing
Nanobioscience
Optical imaging
Optical microscopy
Optical modulation
Optical scattering
Optical signal processing
Particle tracking
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We demonstrate parallel three-dimensional (3D) tracking of multiple fluorescent microspheres with nanometer scale accuracies by engineering the 3D point spread function of a wide-field microscope to present a double-helix along the optical axis.
ISSN:2160-9004
DOI:10.1364/CLEO.2009.CTuAA7