Real-time studies of muscle cell contractions with second harmonic generation microscopy

Real-time second harmonic generation microscopy of live myocytes was achieved with a femtosecond diode-pumped Yb:KGd(WO 4 ) 2 laser. Negligible absorption allows for prolonged imaging during muscle contractions with no significant phototoxic effects or signal degradation.

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Bibliographic Details
Main Authors: Prent, N., Cisek, R., Greenhalgh, C., Major, A., Stewart, B., Barzda, V.
Format: Conference Proceeding
Language:English
Subjects:
(140.3615) Lasers
(170.3880) Medical and Biological Imaging
Absorption
Biomedical optical imaging
Diodes
Frequency conversion
Muscles
Nonlinear optics
Optical harmonic generation
Optical imaging
Optical microscopy
Ultrafast optics
Ytterbium
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Description
Summary:Real-time second harmonic generation microscopy of live myocytes was achieved with a femtosecond diode-pumped Yb:KGd(WO 4 ) 2 laser. Negligible absorption allows for prolonged imaging during muscle contractions with no significant phototoxic effects or signal degradation.
ISSN:2160-9004
DOI:10.1364/CLEO.2009.CWE3