A femtosecond Yb:KGd(WO4)2 laser for biological imaging of yellow fluorescent protein

Real time multiphoton excitation fluorescence microscopy of enhanced yellow fluorescent protein was performed with low photobleaching using a femtosecond Yb:KGd(WO 4 ) 2 laser. Using a conventional Ti:S laser this was previously unfeasible due to low excitation efficiency.

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Bibliographic Details
Main Authors: Sandkuijl, D., Major, A., Cisek, R., Grang, G., Mathur, J., Stewart, B., Barzda, V.
Format: Conference Proceeding
Language:English
Subjects:
(140.3615) Lasers
(180.4315) Nonlinear microscopy
Biomedical optical imaging
Fluorescence
Laser excitation
Nonlinear optics
Optical harmonic generation
Optical imaging
Optical microscopy
Optical pumping
Photobleaching
Ultrafast optics
Ytterbiums
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Description
Summary:Real time multiphoton excitation fluorescence microscopy of enhanced yellow fluorescent protein was performed with low photobleaching using a femtosecond Yb:KGd(WO 4 ) 2 laser. Using a conventional Ti:S laser this was previously unfeasible due to low excitation efficiency.
ISSN:2160-9004
DOI:10.1364/CLEO.2009.CWE5